Özge Ebru DAĞCI VARHAN, Gülşah ÇIKRIKÇI IŞIK, Mustafa EKİCİ, Tuba ŞAFAK, Şeref Kerem ÇORBACIOĞLU, Yunsur ÇEVİK

The Impact of Carbon Monoxide İntoxication on Thiol/Disulfide Hemostasis

Objective: Carbon monoxide (CO) poisoning is an oxidative stress factor. The aim of the study is to evaluate impact of CO intoxication on thiol/disulfide homeostasis (TDH), an important antioxidative system of the body. Methods: This is a prospective study included 84 participants in each group. Blood samples were taken two time in study group (CO intoxication group), before and at the end of the 3rd hour of normobaric oxygen therapy and once in the control group. TDH parameters were studied with an automated assay developed by Erel et al. Statistical analysis done with SPSS program. Results: Among thiol/disulfide homeostasis parameters, in study group native (sh) and total thiol (tt) levels in samples taken at the beginning of the oxygen treatment were significantly higher than sh and tt levels of the control group [Study group sh: 399.70 μmoll−1 (354.50-423.65), tt: 439.1 μmoll−1 (390.9-467.3) and control group sh: 362.95 μmoll−1 (321.95-401.25), tt: 396.1 μmoll−1 (358.5-435), p=0.01 and p<0.001 respectively]. There was no difference between the groups in term of other TDH parameters. TDH parameters were measured after 3-hour normobaric oxygen treatment, and it was shown sh and tt levels were significantly reduced after treatment. Conclusion: Our study demonstrated that among TDH parameters native and total thiol levels were increasing in patients with CO poisoning and those levels were decreasing in time during normobaric oxygen treatment.

Keywords:

carbon monoxide intoxication, Thiol/disulfide homeostasis, oxidative stress, antioxidant systems,

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Reumuth G, Alharbi Z, Houschyar KS, Kim BS, Siemers F, Fuchs PC, Grieb G. Carbon monoxide intoxication: What we know. Burns. 2019;45(3):526-530. doi: 10.1016/j.burns.2018.07.006.
Eichhorn L, Thudium M, Jüttner B. The Diagnosis and Treatment of Carbon Monoxide Poisoning. Dtsch Arztebl Int. 2018;115(51-52):863-870. doi: 10.3238/arztebl.2018.0863.
Ergin M, Caliskanturk M, Senat A, Akturk O, Erel O. Disulfide stress in carbon monoxide poisoning. Clin Biochem. 2016;49(16-17):1243-1247. doi: 10.1016/j.clinbiochem.2016.07.019.
Alkadi H. A Review on Free Radicals and Antioxidants. Infect Disord Drug Targets. 2020;20(1):16-26. doi: 10.2174/1871526518666180628124323.
Liguori I, Russo G, Curcio F, Bulli G, Aran L, Della-Morte D, Gargiulo G, Testa G, Cacciatore F, Bonaduce D, Abete P. Oxidative stress, aging, and diseases. Clin Interv Aging. 2018; 13:757-772. doi: 10.2147/CIA.S158513.
Erel O, Neselioglu S. A novel and automated assay for thiol/disulphide homeostasis. Clin Biochem. 2014;47(18):326-32. doi: 10.1016/j.clinbiochem.2014.09.026.
Akyol S, Erdogan S, Idiz N, Celik S, Kaya M, Ucar F, Dane S, Akyol O. The role of reactive oxygen species and oxidative stress in carbon monoxide toxicity: an in-depth analysis. Redox Rep. 2014;19(5):180-9. doi: 10.1179/1351000214Y.0000000094.
Zhang J, Wu H, Zhao Y, Zu H. Therapeutic Effects of Hydrogen Sulfide in Treating Delayed Encephalopathy After Acute Carbon Monoxide Poisoning. Am J Ther. 2016;23(6):e1709-e1714. doi: 10.1097/MJT.0000000000000290.
Kavakli HS, Erel O, Delice O, Gormez G, Isikoglu S, Tanriverdi F. Oxidative stress increases in carbon monoxide poisoning patients. Hum Exp Toxicol. 2011;30(2):160-4. doi: 10.1177/0960327110388539.
De Wolde SD, Hulskes RH, Weenink RP, Hollmann MW, Van Hulst RA. The Effects of Hyperbaric Oxygenation on Oxidative Stress, Inflammation and Angiogenesis. Biomolecules. 2021;11(8):1210. doi: 10.3390/biom11081210.
Teksam O, Sabuncuoğlu S, Girgin G, Özgüneş H. Evaluation of oxidative stress and antioxidant parameters in children with carbon monoxide poisoning. Hum Exp Toxicol. 2019;38(11):1235-1243. doi: 10.1177/0960327119867751.
Jakubczyk K, Dec K, Kałduńska J, Kawczuga D, Kochman J, Janda K. Reactive oxygen species - sources, functions, oxidative damage. Pol Merkur Lekarski. 2020;48(284):124-127.
Erel Ö, Erdoğan S. Thiol-disulfide homeostasis: an integrated approach with biochemical and clinical aspects. Turk J Med Sci. 2020;50(SI-2):1728-1738. doi: 10.3906/sag-2003-64.
Işler Y, Kaya H. Thiol/disulfide homeostasis in patients treated with normobaric or hyperbaric oxygen for carbon monoxide poisoning. Am J Emerg Med. 2022;59:54-58. doi: 10.1016/j.ajem.2022.06.049.
Bağci Z, Arslan A, Neşelioğlu S. Pediatric Carbon Monoxide Poisoning: Effects of Hyperbaric Oxygen Therapy on Thiol/Disulfide Balance. Pediatr Emerg Care. 2022;38(3):104-107. doi: 10.1097/PEC.0000000000002619.
Ulrich K, Jakob U. The role of thiols in antioxidant systems. Free Radic Biol Med. 2019;140:14-27. doi: 10.1016/j.freeradbiomed.2019.05.035.