Tıbbi Bitkilerde Bulunan Kaempferol’ün LPS ile İndüklenmiş THP-1 Makrofajları Üzerine Etkisi

Giriş: İnflamasyon, immün sisteminin toksik bileşikler, hasarlı hücreler ve patojenlerinde içinde yer aldığı çeşitli faktörler ile tetiklediğinde verdiği biyolojik bir cevaptır. Tıbbi bitkilerden izole edilen etkin maddeler, inflamatuar hastalıkların tedavisinde yeni bir terapötik strateji olarak kullanılırken dikkatle seçilmelidir. Kaempferol tıbbi bitkilerde sebzelerde meyvelerde bolca bulunan bir polifenoldur. Kaempferol anti-kanser, anti-viral, anti-bakteriyel özelliklere sahiptir. Amaç: Bu çalışmada amaç LPS ile indüklenmiş THP-1 makrofaj hücrelerinde kaempferolün hücre canlılığı ve inflamasyonun tedavisi için yapılan çalışmalarda hedef molekül olan NO ve PGE2 düzeyleri üzerine etkisini incelemektir. Bulgular: 2022 yılında yapmış olduğumuz çalışmada LPS ile indüklenmiş THP-1 makrofaj hücrelerine 2,5, 5, 7,5, 10 µM konsantrasyonunda kaempferol uygulandı. Hücre canlılığı, PGE2 düzeyi ve NO üretimi incelendi. Farklı dozlarda kaempferol uygulaması THP-1 makrofaj hücrelerinde hücre canlılığını azaltmadığı belirlendi. Ayrıca LPS ile indüklenen THP-1 makrofaj hücrelerinde 2,5 µM kaempferol uygulaması % NO düzeyini ve 7,5 µM kaempferol uygulaması PGE2 düzeyini istatistiksel olarak anlamlı azaltmıştır. Tartışma ve sonuç: 7,5 µM kaempferol uygulaması makrofaj hücrelerinin proliferasyonu negatif etki göstermeyip hem NO düzeyini hemde PGE2 düzeni azaltıp iyi anti inflamatuvar etki göstermektedir.

Anahtar Kelimeler:

Kaempferol, THP-1, Makrofaj, NO, PGE2, LPS, Macrophage

Effect of Kaempferol in Medicinal Plants on LPS-induced THP-1 Macrophages

Introduction: Inflammation is a biological response of the immune system when triggered by various factors including toxic compounds, damaged cells and pathogens. Active substances isolated from medicinal plants should be chosen carefully when using them as a new therapeutic strategy in the treatment of inflammatory diseases. Kaempferol is a polyphenol abundantly found in medicinal plants, vegetables, fruits. Kaempferol has anti-cancer, anti-viral, anti-bacterial properties. Aim: The aim of this study is to examine the effect of kaempferol on NO and PGE2 levels, which are target molecules in studies for the treatment of inflammation, and cell viability in LPS-induced THP-1 macrophage cells. Result: In 2022, Kaempferol was applied to LPS-induced THP-1 macrophage cells at a concentration of 2.5, 5, 7.5, 10 µM. Kaempferol administration to THP-1 macrophage cells did not affect cell viability and also decreased PGE2 and NO % levels. It was determined that administration of kaempferol at different doses did not decrease cell viability in THP-1 macrophage cells. In addition, in THP-1 macrophage cells induced by LPS, 2.5 µM kaempferol application decreased NO % level and 7.5 µM kaempferol application decreased PGE2 level statistically.Discussion and Conclusion: The application of 7.5 µM kaempferol does not have a negative effect on the proliferation of macrophage cells, but decreases both NO level and PGE2 order and shows good anti-inflammatory effect.

Keywords:

Kaempferol THP-1, , Macrophage, NO, , PGE2, LPS,

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