Ahmet ASLAN, Gürbüz POLAT, Esin ATİK, Muhyittin TEMİZ, Özlen Tubay BAĞDATOĞLU, Nedim ABAN

Deneysel Ülseratif Kolitte Selenyumun Etkinliği

Türkçe Özet:Amaç: Selenyum hücre membranını peroksidlerden koruyarak antioksidan etki gösteren esansiyel bir mineraldir. Oksidatif stres ve hücre hasarının özellikle ülseratif kolitde önemli olduğu bilinmektedir. Selenyumun sıçanlardaki deneysel kolit modeli üzerine etkilerinin araştırılması hedeflendi. Yöntem: Çalışma Mustafa Kemal Üniversitesi Veterinerlik Fakültesi Deneysel Araştırma Laboratuvarında yapılmıştır. Sıçanlarda distal kolit, kolon içine 2 ml %4\'lük asetik asit verilmesi ile oluşturulmuştur. Sıçanlar rastgele üç gruba dağıtılmıştır: Grup I sham, (GI, n=8); Grup II kolit model, hiçbir tedavi almadı (GII, n=8); Grup III, kolit+selenyum (0.2 mg/kg/gün intaperitoneal). Bütün sıçanlar yedinci gün sakrifiye edildi ve distal kolonun 8 cm\'lik kısmı rezeke edildi. Bütün hayvanlardan alınan kolon biyopsileri biyokimyasal ve histopatolojik olarak incelendi. Bulgular: Nitrik oksit düzeyleri GII ile GI ve GIII arasında anlamlı olarak farklı idi (p=0.017 ve p=0.004). Malondialdehit düzeyleri GI ile GII ve GIII arasında anlamlı olarak farklı idi (p=0.001 ve p=0.001). Miyeloperoksidaz düzeyleri GI ile GII ve GIII arasında anlamlı farklılık gösterdi (p=0.001 ve p=0.001). Gruplar arasında katalaz düzeyleri açısından anlamlı bir fark yoktu (p>0.05). Kolon dokusunun histolojik değerlendirmesi sonucu GI\'de normal mukoza, GII\'de mukozal hemoraji, ciddi inflamatuar hücre infiltrasyonu, submukozal ödem ve fokal ülserasyon görüldü. GIII\'de orta derecede nötrofil hücre infiltrasyonu ile üç sıçanda ülserasyon ve diffüz ödem gözlendi. Sonuç: Selenyumun deneysel kolit modelinde iyileşme sürecine olumlu etkileri olduğu sonucuna varıldı.

Anahtar Kelimeler:

selenyum, deneysel kolit, nitrik oksit, malondialdehit

Deneysel Ülseratif Kolitte Selenyumun Etkinliği

Abstract Effect of Selenium in Experimental Ulcerative Colitis Objective: Selenium is an essential biological element which has been shown to have antioxidant effects by protecting the cell membrane from peroxides. It is well known that oxidative stress and cell damage are important especially in UC. We aimed to investigate the effect of selenium in experimental colitis on rats. Method: The study was performed at Mustafa Kemal University Veterinary Faculty Experimental Research Laboratory. Distal colitis of rats was induced by intracolonic instillation of 2 ml of 4% acetic acid. The animals were randomly assigned into three groups: group I as sham, (GI, n=8); group II as colitis model, received no treatment (GII, n=8); group III, colitis+Selenium (0,2 mg/kg /day intaperitoneal). Rats were sacrificed on the 7th day, and the distal 8 cm of the colon was resected. Colonic biopsies from each animal were taken for histopathological examination and biochemical studies. Results: Nitric oxide levels were statistically significant when GII was compared to GIII and GII was compared to GI (p=0.017 and p=0.004). Malondialdehyde levels were significantly different when GI was compared to GII, GI and GIII (p=0.001 and p=0.001). Myeloperoxidase levels were significantly different when GI was compared to GII, GI and GIII (p=0.001 and p=0.001). There was not a statistically significant difference between the groups in catalase levels (p>0.05). Histological evaluation of colonic tissues revealed essentially normal mucosa in GI, in contrast with mucosal haemorrhage, severe inflammatory cell infiltration, submucosal oedema and focal ulceration in GII. In GIII, there was a moderate neutrophyl infiltration with diffuse edema and ulceration in 3 of the 8 rats. Conclusion: It is concluded that selenium has some benefical effects on wound healing in experimental colitis.

Keywords:

selenium, experimental colitis, nitric oxide, malondialdehyde,

PDF

___

Brenneisen P, Steinbrenner H, Sies H. Selenium, oxidative stress, and health aspects. Mol Aspects Med 2005;26(4- ):256—67.
Stapleton SR. Introduction: the selenium conundrum. Cell Mol Life Sci 2000;57(13—14):1823—4.
Rayman MP. The importance of selenium to human health. Lancet 2000; 356(9225):233-41.
Ip C. Lessons from basic research in selenium and cancer prevention. JNutr 1998;128(11):1845—54.
Ozturk C, Avlan D, Cinel I, Cinel L, Unlu A, Camdeviren H, Atik U, Oral U. Selenium pretreatment prevents bacterial translocation in sıçan intestinal ischemia/reperfusion model. Pharmacol Res ;46(2):171-5. Ozardali I, Bitiren M, Karakilcik AZ, Zerin M, Aksoy N, Musa D. Effects of selenium on histopathological and enzymatic changes in experimental liver injury of rats. Exp Toxicol Pathol 2004;56(1—2):59—64.
Podolsky DK. Inflammatory bowel disease. N Engl J Med ;347(6):4l7-29. Kruidenier L, Kuiper I, Lamers CB, Verspaget HW. Intestinal oxidative damage in inflammatory bowel disease: semi—quantification, localization, and association with mucosal antioxidants. J Pathol 2003;201(l):28—36.
Schrauzer GN. Anticarcinogenic effects of selenium. Cell Mol Life Sci 2000;57(13- 14): 1864-73.
Advances in Animal Nutrition. Oxford: Butterworth and Heinemann’s;l994:3—30. of the common cranberry Vaccinium oxycoccos L. on acetic acid—induced colitis in mice. World J Gastroenterol ;12(41):6646—51. protects the intestine and reduces bacterial translocation. Shock 2002;18(5):476—80. ;108:107—10.
Aebi H. Catalase. In: Bergmayer HV ed. Methods of Enzymatic Analysis. NY and London, Academic Pres Inc Grisham MB, Hernandez LA, Granger DN. Xanthine oxidase and neutrophil infiltration in intestinal ischemia. Glowick SP, Kaplan SP. Methods in Enzymology. New York, Academic Pres, 1955:769—82.
Kruidenier L, Verspaget HW. Oxidative stress as a pathogenic factor in inşammatory bowel disease radicals or ridiculous? Aliment Pharmacol Ther 2002;16(12):1997—
Kruidenier L, Kuiper I, van Duijn W, Marklund SL, van Hogezand RA, Lamers CB, Verspaget HW. Differential Tuzun A, Erdil A, Inal V, Aydin A, Bagci S, Yesilova Z, Sayal A, Karaeren N, Dagalp K. Oxidative stress and Kruidenier L, Kuiper 1, Van Duijn W, Mieremet-Ooms MA, van Hogezand RA, Lamers CB, Verspaget HW. Imbalanced secondary mucosal antioxidant response in inşammatory bowel disease. J Pathol 2003;201(1):17—27.