Arı zehrinin anti-tümör etkenliğinde olası mekanizmalar olarak apoptosis ve nekrosis

Arı zehirinin başlıca bileşenlerinden olan melitinin, kalmodülin etkinliğini durdurmada çok etkili olduğu ve hücre büyümesi ile klonogenijite açısından da aynı durdurucu etkiyi gösterdiği gözlemlerinden hareketle, arı zehirinin olası tümör büyümesi ve metastaz süreçlerini durdurucu etkileri in vivo (canlı dokularda) ve in vitro (deney tüpünde) olarak araştırılmıştır. Kullanılan tümör, aktarılabilen pirimer meme kanseriydi (MCa). Başlangıçtaki tümör 1x$10^5$ tümör hücreleri ile elde edilirken, $10^5$ tümör hücrelerinin damar içine zerk edilmesiyle akciğerlerde metastaz yaratılmıştır. Arı zehiri, duruma göre, farelerin deri altlarına (sc) 150, 300, 600 $mu$g/fare ya da damarlarına (iv) 75 ve 150 $mu$g/fare dozlarında verilmiştir. Tümör hücreleri arı zehirinden hemen sonra zerk edildiğinde, kullanılan arı zehiri dozu ne olursa olsun tümör büyümesi hemen durdurulmuş, bu anlamda tedavi gören farelerin hayatta kalış süreleri de uzamıştır. Damarlardan arı zehiri alan farelerde akciğer metastaz sayısı, kontrol grubundakine göre önemli ölçüde daha azdır (p

Apoptosis and necrosis as possible mechanisms for antitumor activity of bee venom

Based on the observation that melittin, a major component of bee venom, is a very potent inhibitor ofcalmodulin activity and a potent inhibitor of cell growth and clonogenicity, possible tumor growth- and metastasis-inhibiting effects of bee venom in vivo or in vitro were studied. Tumor was a transplantable mammary carcinoma (MCa) of spontaneous origin. Tumor in the footpad was generated by 1x$10^5$ tumor cells, while intravenous (iv) injection $10^5$ tumor cells generated métastases in the lungs. Bee venom was injected into mice subcutaneously (sc) at doses of 150, 300, 600 $mu$g/mouse or 75 and 150 fig/mouse intravenously (iv), respectively. When tumor cells were injected into footpad immediately after bee venom, the growth of tumor was suppressed irrespective of the venom dose administered. The survival of treated mice was also prolonged. The number of lung métastases in mice treated i v with bee venom was significantly lower (p

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