Mehmet Akif KAHRAMAN, Halil İbrahim GÖKÇE

Felin İnfeksiyöz Peritonitli Kedilerde Adenozin Deaminaz ve C-Reaktif Protein Düzeylerinin Araştırılması

Investigations of Adenosine Deaminase and C-reactive Protein in Cats with Feline Infectious Peritonitis

Çalışmada felin infeksiyöz peritonitli (FİP) kedilerde serum adenozin deaminaz-1 (ADA-1), C-reaktif protein (CRP) ve albümin (A) seviyelerini ölçerek T-lenfosit aracılı immun yanıt ve akut faz yanıtın değerlendirilmesi amaçlanmıştır. Çalışmada 20 FİP’li ve 10 adet klinik olarak sağlıklı kedi kullanıldı. Ayrıca kediler klinik, röntgen ve nekropsi bulguları ışığında yaş form (n=10) ve kuru form (n=10) FİP’li olarak iki gruba ayrıldı. Kedilerin serum ADA-1 ve CRP düzeyleri kedi spesifik ELISA testleri kullanılarak belirlendi. Serum total protein (TP) ve albümin düzeyleri ise fotometrik metodla saptandı. Çalışmada FİP’li kedilerin hem serum hem de peritoneal efuzyonlarındaki ADA-1 (p<0,001) ve CRP (p<0,001) düzeyleri sağlıklı kedilerin düzeylerine göre istatistiksel olarak anlamlı düzeyde yüksek olduğu belirlendi. FİP’li kedilerin serum ADA-1 konsantrasyonu ile CRP konsantrasyonları arasında yüksek düşeyde pozitif korelasyonun olduğu belirlendi (r=0,62, p<0,001). Ayrıca FİP’li kedilerin serum TP (p<0,01) ve globülin (G) (p<0,01) düzeylerinde anlamlı artışlar belirlenirken albümin (p<0,01) düzeyleri ve albümin globülin oranlarında (A/G) ise sağlıklı kedilere göre anlamlı düzeyde düşüşlerin olduğu saptandı. Kuru form FİP’li kedilerin serum TP (p<0,05), ve G (p<0,05) düzeyleri yaş form FİP’li kedilerinkiyle karşılaştırıldığında anlamlı düzeyde yüksek iken ADA-1 p<0,01) aktivitesinin ise anlamlı düzeyde düşük olduğu saptandı. Sonuç olarak, FİP’li kedilerde artan serum ADA-1 aktivitesi T-lenfosit aktivasyonunu ve artan CRP ile düşen albümin ise akut faz yanıtın geliştiğini ortaya koymaktadır. FİP’li kedilerde hem serum hem de peritoneal efüzyonların ADA-1 ve CRP düzeylerinin ölçümünde kullanılabileceği ve bu parametrelerin FİP’li kedilerde T-lenfosit aracılı immun yanıt, yangı ve olası organ hasarlarının değerlendirilmesinde yararlı biyomarkırlar olduğu düşünülmektedir. Anahtar Kelimeler: Adenozin Deaminaz-1 (ADA-1), C-Reaktif Protein (CRP) Felin İnfeksiyöz Peritonitis (FIP), Kedi.

Keywords:

Adenosine Deaminase-1 (ADA-1), Cat, C-Reactive Protein (CRP) , Feline Infectious Peritonitis (FIP).,

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