Meme Kanseri Hücrelerinde Zamana Bağlı HIF-2α İfadesi Değişiminin Analizi

Meme kanseri kadınlarda en sık rastlanan kanser türüdür veTürkiye’de gözlemlenen vaka sayısı her geçen gün artmaktadır. Meme kanserinin içinde yer aldığı birçok kanser türünde, kanserli doku kan damarlarından uzak bölgelerde büyürken oksijen eksikliğine maruz kalır. Hipoksiya olarak adlandırılan bu duruma karşı dokular Hipoksi ile indüklenebilir faktör (HIF) transkripsiyon faktörlerinin ifade artışı ile cevap verirler. HIF transkripsiyon faktörlerinin etkisiyle kanserli hücreler, damar gelişimini uyararak farklı doku ve organlara göç ederler. Bu nedenle HIF transkripsiyon faktörlerinin hipoksiya sürecindeki değişiminin bilinmesi önemlidir. HIF-2α hipoksik koşullar altında ifadesi artan bir transkripsiyon faktörüdür ve farklı kanser türlerinde ifade düzeyi değişiklik göstermektedir. Bu çalışmanın amacı farklı meme kanseri ve normal meme epiteli hücrelerinde farklı sürelerde hipoksiya uygulamasının HIF-2α ifadesinin değişimi üzerinde etkisinin araştırılmasıdır.

Anahtar Kelimeler:

Meme kanseri, hipoksiya, HIF-2a

Analysis of Time-Dependent Alteration of HIF-2α Expression in Breast Cancer Cells

Breast cancer is the most commonly diagnoised cancer type in women and the number of cases observed in Turkey has increased day by day. In many types of cancer including breast cancer, tumor tissues are exposed to oxygen deficiency while growing in areas distant from blood vessels. Against this condition, knew as hypoxia, tissues respond via increasing the expression of transcription factors called Hypoxia inducible factor (HIF). By the effect of HIF transcription factors, cancer cells migrate to different tissues and organs by stimulating vascular development. Therefore, it is important to know the change of HIF transcription factors in hypoxia process. HIF-2α is a transcription factor, which is over expressed during hypoxic conditions and have varies the level of expression in different cancer types. The aim of this study is that to investigate the effect of time dependent hypoxia exposure on HIF-2α expression in different breast cancer cells and normal breast epithelial cells.

Keywords:

Breast cancer, hypoxia, HIF-2α,

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