Histological changes in juvenile common carp (Cyprinus carpio L.) fed on high levels of chromium chloride hexahydrate
Histological changes in juvenile common carp (Cyprinus carpio L.) fed on high levels of chromium chloride hexahydrate
We designed this experiment to examine the impact of high levels of dietary Cr(III) on the histological structure of the gut, liver, kidney and spleen of the juvenile common carp (Cyprinus carpio L.; average weight 15 ± 0.69 g). Fish were fed on different levels of dietary Cr as chromium chloride CrCl3.6H2O (0.0, 4.0, 6.0 and 8.0 mg) Cr/kg diet for 10 weeks at 25 ± 1.5ºC. At the end of the experiment, no mortalities were recorded. High levels of dietary Cr impaired the normal histological structure of the tested organs. Different Cr treatments showed signs of toxicity in the liver and gut that involved the appearance of foci of inflammatory cells, pyknotic nuclei, foci of melanomacrophages, vacuole formation, degeneration of hepatocytes and erythrocytes aggregation in the liver and lifting of the lining epithelium, vacuolation, surface erosion in the intestine. The kidney of Cr treatments showed remarkable changes that include renal tubular separation, cytoplasmic vacuolation, aggregation of red blood cells, degeneration of renal tubules, necrosis of hematopoietic tissue and oedema. Injuries and damage were observed in the spleens of fish fed with Cr-fortified diets, including necrosis, depletion of lymphoid tissues and vacuoles formation. In conclusion, ingestion of high levels of dietary Cr (III) generated adverse health effects, and the application of Cr (III) in fish diets should be cautious.
Keywords:
Chromium chloride , , histopathology, toxicity, , common carb ( Cyprinus carpio L.),
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- Ahmed, A. R., Jha, A. N., Davies, S. J. (2012a). The efficacy of chromium as a growth enhancer
- Ahmed, A. R., Jha, A. N., Davies, S. J. (2012b). The effect of dietary organic chromium on
- Ahmed, A. R., Moody, A. J., Fisher, A., Davies, S. J. (2013). Growth performance and starch utilization
- Anderson, R. A. (1995). Chromium, glucose tolerance, diabetes and lipid metabolism. J Adv Med, 8, 37-49.
- Bakshi, A., Panigrahi, A. K. (2018). A comprehensive review on chromium induced alterations in fresh water fishes. Di Giulio, R.T., Hinton, D.E. (2008). The toxicology of fishes. London and New York, CRC Press.
- Gallhoefer, N. S., Bentley, E., Ruetten, M., Grest, P., Haessig, M., Kircher, P. R., Dubielzig, R.R., Spiess, B.M., Pot, S. A. (2013). Comparison of Gatta, P. P., Piva, A., Paolini, M., Testi, S., Bonaldo, A., Antelli, A., Mordenti, A. (2001). Effects of dietary organic
- Genten, F., Terwinghe, E., Danguy, A. (2009). Atlas of Fish Histology. Science publishers, Enfield, NH, USA.
- Giri, A. K., Sahu, N. P., Saharan, N., Dash, G. (2014). Effect of dietary supplementation of chromium on Gorgieva, E., Arnaudov, A., Velcheva, I. (2010). Clinical, hematological and morphological
- Hinton, D.E., Segner, H., Braunbeck, T. (2001) Toxic responses of the liver. In: Schlenk, D., Benson, W.H. (Eds).
- Lawrence, A.J., Hemingway, K.L. (2003). Effects of pollution on fish: Molecular effects and
- Li, S., Zhou, S., (2008). Side-effect of pesticides on aquatic organisms In Svensson, E. P.
- Liu, T., Wen, H., Jiang, M., Yuan, D., Gao, P., Zhao, Y., Wu, F., Liu, W. (2010). Effect of dietary chromium picolinate
- Mehrim, A. I. (2014). Physiological, biochemical and histometric responses of Nile tilapia Mishra, A. K., Mohanty, B. (2008). Acute toxicity impacts of hexavalent chromium on behavior Milner, J. A. (1990). Trace minerals in the nutrition of children. The Journal of pediatrics, 117(2), S147-S155. Mohamed, A. A. R., El-Houseiny, W., Abd Elhakeem, E. M., Ebraheim, L. L., Ahmed, A. I., Abd El-Hakim, Y. M. (2020). Effect of hexavalent
- Muthukumaravel, K. and Rajaraman, P. (2013) A Study on the toxicity of chromium on the histology
- Musumeci, G. (2014). Past, present and future: overview on histology and histopathology. Journal of
- Ozawa, T., Hanaki, A. (1990). Spin-trapping studies on the reactions of Cr (III) Pan, Q. B. I. Y. Z., Bi, Y. Z., Yan, X. L., Pu, Y. Y., Zheng, C. (2002). Effect of organic chromium on
- Pechova, A., Pavlata, L. (2007). Chromium as an essential nutrient: a review. VETERINARNI MEDICINA-PRAHA-, 52(1), 1.
- Pizzino, G., Irrera, N., Cucinotta, M., Pallio, G., Mannino, F., Arcoraci, V., Squadrito, F., Altavilla, D., Bitto, A. (2017). Rakhmawati , R.; Suprayudi, M.A; Setiawati, M.; Widanarni, W.; Junior, M.Z.; and Jusadi, D. 2018. Bioefficacy Selcuk, Z., Tiril, S.U., Alagil, F., Belen, V., Salman, M., Cenesiz, S., Muglali, O.H. Yagci, F.B. (2010). Effects of dietary
- Stearns, D. M., Belbruno, J. J., Wetterhahn, K. E. (1995). A prediction of chromium (III)
- Tacon, A.G.J, Beveridge, M.M. (1982) Effects of dietary trivalent chromium on rainbow trout,
- Velma, V. , Tchounwou, P.B. (2013). Oxidative Stress and DNA Damage Induced by Chromium in Liver and
- Zafra-Stone, S., Bagchi, M., Preuss, H.G., Bagchi, D. (2007) Benefits of chromium (III)
- Başlangıç: 2018
- Yayıncı: Mersin Üniversitesi
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