Complete blood count (CBC) is one of the most common laboratory tests. Preanalytical factors like specimen homogenization affect the CBC test results. In the emergency laboratory, test time is a ciritcal parameter. Optimization of test procedures is of critical importance to give more accurate results in a shorter time period. This study was designed to evaluate the effects of preanalytical mechanical mixing times on CBC parameters and to optimize the mechanical mixing time for the analysis. Mechanical mixing time of 1 and 5 minutes (t= 1 and t=5 respectively) with a rotary type mixer and automatic mixing performed by the Coulter LH 780 (t=0) prior to complete blood count analysis were evaluated. Between t=0 and t=1 minutes of mechanical mixing, only a significant change in MPV values were obtained (p
___
1. Gay-George B, Parker K. Understanding the complete blood count with differential. J Perianesth Nurs. 2003;18(2):96-117.
2. Zandecki M, Genevieve F, Gerard J, Godon A. Spurious counts and spurious results on hematology analysers: a review. Part II: white blood cells, red blood cells, haemoglobin, red cell indices and reticulicytes. Int Jnl Lab Hem. 2007;29:21-41.
3. Narayanan S. Preanalytical issues in hematology. J Lab Med. 2003;27:243-48.
4. Ricos C, Alvarez V, Cava F, Garcia-Lario JV, Hernandez A, Jimenez CV, Minchinela J, Perich C, Simon M. Current databases on biological variation: pros, cons and progress (2014 update) Scand L Clin Lab Invest. 1999;59:491-500.
5. Cantero M, Conejo JR, Jimenez A. Interference from lipemia in cell count by hematological analyzers. Clin Chem. 1996;42:987-88.
6. Lippi G, Salvagno GL, Solero GP, Montagnana M, Guidi GC. The influence of tourniquet time on hematological testing for antidoping purposes. Int J Sports Med. 2006;27:359-62.
7. Banfi G, Germagnoli L. Preanalytical Phase in Haemotology. JMB. 2008;27:348-53.
8. Lippi G, Salvagno GL, Solero GP, Montagnana M, Banfi G, Guidi GC. Evaluation of different mixing procedures for K2EDTA primary samples on haemotological testing. Lab-medicine. 2007;38:723-5.
9. Pewarchuk W, Vanderbroom J, Blacjchman MA. Pseudopolycythemia, pseudothrombocytopenia, and pseudoleukopenia due to overfilling of blood collection vacuum tubes. Arch Pathol Lab Med. 1992;116:90-2.
10. Neville RG. Evaluation of portable hemoglobinometer in general practice. Br J Med. 1987;294:1263-7.
11. Lima-Oliveira G, Lippi G, Savagno GL, Brocco G, Gaino S, Dima F, Volaski W, Rego FG, Picheth G, Guidi GC. Processing of diagnostic blood specimens: is it really necessary to mix primary blood tubes after collection with evacuated tube system? Biopreserv Biobank. 2014;12(1):53-9.
12. Ashenden M, Clarke A, Sharpe K, d’Onforio G, Allbon G, Gore CJ. Preanalytical mixing of whole-blood specimens in context of the athlete passport. J Clin Pathol. 2011;200269-online article
13. Lippi U, Schinella M, Modena M, Nicoli M. A new anticoagulant solution to stabilize platelet indices. Progr Med Lab. 1989;3:111-5.
14. Turhan T, Sezer S, Yücel Ç, Koca Y. Effects of storage conditions on complete blood cell count parameters. Turk J Biochem. 2011;36(2):165-74.