HACİ AHMET DEVECİ, GÖKHAN NUR, HÜLYA ÇİÇEK, MAHMUT KARAPEHLİVAN

Evaluation of oxidative stress factors in patients with osteoporosis

Osteoporosis is an important disease with an increasing incidence that leads to serious losses in physical function and adversely affecting the life quality of women especially in postmenopausal period. Age, genetic structure, vitamin D and K abnormalities, estrogen deficiency, chronic inflammation and oxidative stress are considered among the most important risk factors for osteoporosis. Therefore, we aimed to investigate the plasma paraoxonase (PON1) activity, highdensity lipoprotein (HDL), total sialic acid (TSA) and total oxidant/antioxidant status (TOS/TAS) of patients with osteoporosis in this study. Comparisons of 25 female patients diagnosed with osteoporosis aged between 51-67 and 10 healthy women between the ages ranged 50-68 were made in this study. PON1 activity, HDL, TSA, TOS and TAS levels were measured by spectrophotometrical method in plasma samples that obtained from blood samples of patients and healthy individuals. Results were expressed as mean ± standard deviation. Plasma PON1 activity, TAS and HDL levels were significantly lower in patiens with osteoporosis than the healty group (p

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1. Kanis JA. Osteoporosis and its consequences. in: Kanis JA eds, Osteoporosis. BlackwelJ Science Ltd. London. 1997;21.
2. Biberoğlu S. Pathogenezis of osteoporosis. Kutsal Y.G. Osteoporoz. 2nd edition. Ankara/Turkey: Güneş. 2005;37-60.
3. Akıncı A, Bartl R, Frisch B. Osteoporosis. 1st eds. Ankara: Turkish Clinics. 2006;1-24.
4. Merly SL. Metabolic bone diseases. in: Kelley WH, Harris ED, Ruddy S, Sledge CB, eds, Textbook of Rheumatology. WB Saunders Company, Philadelphia. 1997;563-1581.
5. Ersler WB, Harman SM, Keller ET. Immunologic aspects of osteoporosis. Dev Comp Immunol. 1997;21(6):487-99.
6. Çavdaroğlu B, Köse N, Başkol G, Demir H. Postmenopozal osteoporozlu hastalarda proteinler ve lipidlerdeki oksidatif stresin değerlendirilmesi. Dicle Tip Fakul Derg. 2014;41(1):71-77.
7. Yang S, Ries WL, Key LL. Nicotinamide adenine dinucleotide phosphate oxidase in the formation of superoxide in osteoclasts. Calcif Tissue Int. 1998;63(4):346-50.
8. Kutlu R, Çivi S, Pamuk G. Postmenopozal kadınlarda osteoporoz sıklığı ve FRAXTM Skalası kullanılarak 10 yıllık kırık riskinin hesaplanması. Türk Fiz Tıp Rehab Derg. 2012;58:126-35.
9. Tabakoğlu E,, Durgut R. Veteriner hekimlikte oksidatif stres ve bazı önemli hastalıklarda oksidatif stresin etkileri. AVKAE Derg. 2013;3(1):69-75.
10. Basu S, Michaelsson K, Olofsson H, Johansson S, Melhus H. Association between oxidative stress and bone mineral density. Biochem Biophys Res Commun. 2001;288(1):275-9.
11. Sontakke AN, Tare RS. A duality in the roles of reactive oxygen species with respect to bone metabolism. Clin Chim Acta. 2002;318(1-2):145-8.
12. Madenci E, Güçbey Ş, Koca I, Ibas E, Çiçek H. Serum total oxidative and antioxidative status in patients with postmenopausal osteoporosis. Nobel Medicus. 2015;11(1):83-86.
13. Eckerson HW, Romson J, Wyte CM, La Du BN. The human serum paraoxonase polymorphism: Identification of phenotypes by their response to salts. Am J Hum Genet. 1983;35(2):214-27.
14. Gülcü F, Gürsu MF. Paraoksonaz ve aril esteraz aktivite ölçümlerinin standardizasyonu. Turk J Biochem. 2003;28:45-9.
15. Erel O. A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable abts radical cation. Clin Biochem. 2004;37(4):277-85.
16. Erel O. A new automated colorimetric method for measuring total oxidant status. Clin Biochem. 2005;38(12):1103-11.
17. Sydow G. A simplifield quick method for determination of sialic acid in serum. Biomed Biochem Acta. 1985;44(11-12):1721-3.
18. Verit FF, Celik H, Yazgan P, Erel O, Geyikli I. Paraoxonase 1 activity as a marker of atherosclerosis is not associated with low bone mineral density in healthy postmenopausal women. Arch Gynecol Obstet. 2007;275(5):353-9.
19. Yamada Y, Ando F, Niino N, Miki T, Shimokata H. Association of polymorphisms of paraoxonase 1 and 2 genes, alone or in combination, with bone mineral density in community-dwelling Japanese. J Hum Genet. 2003;48:469-75.
20. Karataş F, Ozates I, Canatan H, Halifeoğlu I, Karatepe M, Colakt R. Antioxidant status and lipid peroxidation in patients with rheumatoid arthritis. Indian J Med Res. 2003;118:178-81.
21. Bekpınar S, Kilic N, Unlucerci Y, Akdağ-Köse A, Azizlerli G, ÖzbekKir Z. Evaluation of nitrosative and oxidative stress in Behçet disease. J Eur Dermatol Venereol. 2005;19(2):167-71.
22. Altındağ O, Erel O, Aksoy N, Selek S, Çelik H, Karaoğlanoğlu M. Increased oxidative stress and its relation with collagen metabolism in knee osteoarthritis. Rheumatol Int. 2007;27(4):339-44.
23. Ozgocmen S, Kaya H, Fadillioğlu E, Aydoğan R, Yılmaz Z. Role of antioxidant systems, lipid peroxidation, and nitric oxide in postmenopausal osteoporosis. Mol Cell Biochem. 2007;295(1-2):45- 52.
24. Sanchez-Rodriguez M, Ruiz-Ramos M, Correa-Munoz E, MendozaNunez VM. Oxidative stress as a risk factor for osteoporosis in elderly Mexicans as characterized by antioxidant enzymes. BMC Musculoskeletal Disorders. 2007;8:124.
25. Altindag O, Erel O, Soran N, Celik H, Selek S. Total oxidative/antioxidative status and relation to bone mineral density in osteoporosis. Rheumatol Int. 2008;28(4):317-21.
26. Deyhim F, Garica K, Lopez E, Gonzales J, Ino S, Garcia M, Patil BS. Citrus juice modulates bone strength in male senescent rat model of osteoporosis. Nutrition. 2006;22(5):559-63.