Zeynel Han, Tamer Erdem, Nasuhi Engin Aydın

Effect of Cigarette Smoke on Rabbit Ear Cartılage Graft Viability and Integrity [Sigara Dumanının Tavşan Kulak Kıkırdak Grefti Canlılık ve Bütünlüğüne Etkisi]

AbstractThe effect of cigarette smoking on cartilage grafts implanted to rabbit ear is investigated on New Zealand White rabbits. Eight animals for each experimental and control groups were maintained. Cartilage grafts were taken from the left ear and implanted as smaller pieces to paraspinal soft tissues bilaterally in each animal. Cartilage fragments were implanted as intact, crushed for once or twice to the six paraspinal soft tissue sites.The duration of the experiment was 12 weeks. One group was exposed to cigarette smoke in a special chamber for 4 hours a day continuously during this period. Cartilage grafts on one paraspinal site were examined microscopically. At the end of the 12th week the grafts on the other side were all excised and examined microscopically in respect to viability, sclerosis, osteoid formation and regeneration. Statistically significant differences were not found between the groups except intact and crushed grafts on the 12 week smoking model in regard to viability which was less in the crushed cartilages. Our finding in this experimental model suggests a negative effect of cigarette smoking on crushed cartilage grafts which opens a door to further experimental models and clinical observations. Key words: Cigarette smoke, cartilage graft viability, experimental model

Anahtar Kelimeler:

-

Keywords:

Cigarette smoke, cartilage graft viability experimental model,

PDF

___

Kaynaklar
Yıldız L, Kılıç H. Sigaranın klinik ve biyokimyasal etkileri, Türkiye Klinikleri Tıp Bilimleri. 2000;20(5):306-12.
Golding JF. Smoking. In: Gibson GJ, Geddes DM, Cosabel U, Sterk PJ, Corrin B, eds, Respiratory Medicine, 3rd revised edition. Saunders; 2003;445-60.
Silverstein P. Smoking and wound healing. Am J Med. 1992;93(1A):22-4.
Amin S, Niu J, Guermazi A, Grigoryan M, Hunter DJ, Clancy M, LaValley MP, Genant HK, Felson DT. Cigarette smoking and the risk for cartilage loss and knee pain in men with knee osteoarthritis. Ann Rheum Dis. 2007;66(1):18-22.
Kanneganti P, Harris JD, Brophy RH, Carey JL, Lattermann C, Flanigan DC. The effect of smoking on ligament and cartilage surgery in the knee. Am J Sports Med. 2012;40(12):2872-8.
Uematsu Y, Matuzaki H, Iwahashi M. Effects of nicotine on the intervertebral disc: an experimental study in rabbits. J Orthop Sci. 2001;6(2):177-82.
Iwahashi M, Matsuzaki H, Tokuhashi Y, Wakabayashi K, Uematsu Y. Mechanism of intervertebral disc degeneration caused by nicotine in rabbits to explicate intervertebral disc disorders caused by smoking. Spine. 2002;27(13):1396-401.
Kazikdas KC, Ergur B, Tugyan K, Guneli E, Kaya D, Sahan M. Viability of crushed and diced cartilage grafts wrapped in oxidized regenerated cellulose and esterified hyaluronic acid: an experimental study. Laryngoscope. 2007;117(10):1728-34.
Lattyak BV, Maas CS, Sykes JM. Dorsal onlay cartilage autografts: comparing resorption in a rabbit model. Arch Facial Plast Surg. 2003;5(3):240-3.
Erol, OO. The Turkish delight: A pliable graft for rhinoplasty. Plast Reconstr Surg. 2000;105(6):2229-41.
Daniel RK1, Calvert JW. Diced cartilage grafts in rhinoplasty surgery. Plast Reconstr Surg. 2004;113(7):2156-71.
Cakmak O, Buyuklu F, Yilmaz Z, Sahin FI, Tarhan E, Ozluoglu LN. Viability of cultured human nasal septum chondrocytes after crushing. Arch Facial Plast Surg. 2005;7(6):406-9.
Rudderman RH, Guyuron B, Mendelsohn G. The fate of noncrushed and crushed autogenous cartilage in the rabbit model. Ann Plast Surg. 1994;32(3):250-4.
Cakmak O, Bircan S, Buyuklu F, Tuncer I, Dal T, Ozluoglu LN. Viability of crushed and diced cartilage grafts. Arch Facial Plast Surg. 2005;7(1):21-6.
Verwoerd-Verhoef HL, Meeuwis CA, van der Heul RO, Verwoerd CD. Histologic evaluation of crushed cartilage grafts in the growing nasal septum of young rabbits. ORL J Otorhinolaryngol Relat Spec. 1991;53(5):305-9.