This study was designed to evaluate the phenolic profiles and antioxidant potentials of two commercial Crataegus monogyna Jacq samples, which are commonly used for various medicinal purposes. One of the samples supplied from the pharmacy and the other sample supplied from the herbalist shop. To analyze phenolic contents of samples Total Phenolic Content, Total Flavonoid Content and Total Phenolic Acid Content Assays were carried out. Further hyperoside contents, which is one of the major component of the genus, were determined by HPTLC method. Antioxidant bioactivities were established by using CUPRAC (Cupric Reducing Antioxidant Capacity), FRAP (Ferric Reducing Antioxidant Power), DPPH radical scavenging activity and TOAC (Total Antioxidant Capacity) methods. The comparison between two samples showed that the pharmacy product has higher phenolic ingredients than herbalist shop, consequently, has higher antioxidant bioactivity.
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1. Pittler MH, Schmidt K, Ernst E. Hawthorn extract for treating chronic heart failure. (Protocol) Cochrane Database of Systematic Reviews. 2005;2:CD005312.
2. Venskutonis PR. Phytochemical composition and bioactivities of hawthorn (Crataegus spp.): a review of recent research advances. J. Food Bioact. 2018;4:69–87.
3. Čopra-Janićijević A, Čulum D, Vidic D, et al. Chemical composition and antioxidant activity of the endemic Crataegus microphylla Kochsubsp. malyana K. I. Chr. & Janjić from Bosnia. Ind Crop Prod. 2018; 113:75-9.
4. Wen L, Guo X, Liu RH, et al. Phenolic contents and cellular antioxidant activity of Chinese hawthorn “Crataegus pinnatifida.” Food Chem. 2015;186:54–62.
5. Singleton VL, Rossi JA. Colorimetry of total phenolics with phosphomolybdic phosphotungstic acid reagents. Am J Enol Vitic. 1965;16:144-58.
6. Celep E, Aydın A, Yesilada E. A comparative study on the in vitro antioxidant potentials of three edible fruits Cornelian cherry, Japanese persimmon and cherry laurel. Food Chem Toxicol. 2012;50:3329-35.
7. Mihailovic V, Kreft S, Benkovic ET, et al. Chemical profile, antioxidant activity and stability in stimulated gastrointestinal tract model system of three Verbascum species. Ind Crop Prod. 2016;89:141-51.
8. Cretu G, Morlock G, Miron AR, et al. A high-performance thin-layer chromatographic method for chlorogenic acid and hyperoside determination from berry extracts. Rom Biotechnol Lett. 2013;18:8657-65.
9. Apak R, Güçlü K, Özyürek M, et al. Novel total antioxidant capacity index for dietary polyphenols and vitamins C and E, using their cupric ion reducing capability in the presence of neocuproine: CUPRAC method. J Agric Food Chem. 2004;52:7970–81.
10. Benzie IFF, Strain, JJ, The ferric reducing ability of plasma (FRAP) as a measure of antioxidant power: the FRAP assay. Anal Biochem. 1996;239:70- 6.
11. Prieto P, Pineda M, Aguilar M. Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E. Anal Biochem. 1999;269:337– 41.
12. Celep E, Aydın A, Kırmızıbekmez H, et al. Appraisal of in vitro and in vivo antioxidant activity potential of cornelian cherry leaves. Food Chem Toxicol. 2013;62:448–55.
13. Elvin-Lewis M. Safety issues associated with herbal ingredients. Adv Food Nutr Res. 2005;50:219-313.
14. Ndhala AR, Moyo M, Van-Staden J. Natural antioxidants: fascinating or mythical biomolecules? Molecules. 2010;15:6905-30.
15. Cosmulescu S, Trandafir I, Nour V. Phenolic acids and flavonoids profiles of extracts from edible wild fruits and their antioxidant properties. Int J Food Prop. 2017;20:3124-3134.
16. Bor Z, Arslan R, Bektaş N, et al. Antinociceptive, anti-inflammatory, and antioxidant activities of the ethanol extract of Crataegus orientalis leaves. Turk J Med Sci. 2012;42:315-24.
17. Ruiz-Rodriguez BM, Ancos B, Sanchez-Moreno C, et al. Wild blackthorn (Prunus spinosa L.) and hawthorn (Crataegus monogyna Jacq.) fruits as valuable sources of antioxidants. Fruits. 2013;69:61-73.
18. Bardakci H, Celep E, Gözet T, et al. Phytochemical characterization and antioxidant activities of the fruit extracts of several Crataegus taxa. S Afr J Bot. 2019;124:5-13