Hilal YILDIRIM, Sedat YILDIZ, Tuba ÖZGÖÇER

Prenatal Ve Postnatal Endotoksin Maruziyetleri: Dişi Sıçanlarda Lökosit Oranları Ve IL-1β, TNF-α, Gonadotropin Ve Kortikosteron Seviyeleri Üzerindeki Etkileri

Amaç: Bu çalışmanın amacı doğum öncesi ve doğum sonrası endotoksin maruziyetinin dişi sıçanlarda hematolojik parametreler, kan sitokinleri (TNF-α, IL-1β), gonadotropinler ve kortikosteron seviyeleri üzerindeki uzun vadeli etkilerini araştırmaktır. Materyal ve Metot: Gebe sıçanlara gebeliğin 17-18. günlerinde intraperitoneal olarak steril salin (SF) veya endotoksin (LPS) enjekte edildi. Doğumdan sonra dişi yavrular iki gruba ayrılarak doğum sonrası 60. günde steril salin (SF, n= 17) veya endotoksin (LPS, n= 17) enjekte edildi ve dört deney grubu oluşturuldu (SF+SF, SF+LPS, LPS+SF ve LPS+LPS). Son enjeksiyondan 4 saat sonra kan örnekleri alındı. Plazma IL-1β, TNF-α, kortikosteron, LH, FSH düzeyleri ve kan lökosit oranları değerlendirildi. Bulgular: Nötrofil % oranı SF+LPS, LPS+SF ve LPS+LPS gruplarında SF+SF grubuna göre daha yüksek ancak lenfosit % oranı daha düşüktü. Kortikosteron, LH ve FSH düzeyleri gruplar arasında farklı değildi ancak TNF-α düzeyleri LPS+LPS gruplarında SF+SF ve LPS+SF gruplarından daha yüksekti. SF+LPS grubunun IL-1β düzeyi SF+SF ve LPS+SF gruplarından daha yüksekti.Sonuç: Prenatal ve post-pubertal endotoksin maruziyetinin, hipotalamo-hipofiz-adrenal ve -gonadal eksenleri etkilemeden sitokin düzeyi, nötrofil ve lenfosit yüzdelerini programladığı belirlenmiştir.

Pre- and Post-Natal Endotoxin Exposures: Effects on Leucocyte Ratios and Levels of IL-1β, TNF-, Gonadotropins and Corticosterone in Female Rats

Aim: The aim of this study is to investigate the long-term effects of prenatal and postnatal endotoxin exposure on hematological parameters, blood cytokines (TNF-α, IL-1β), gonadotropins and corticosterone levels in female rats. Material and Method: Pregnant rats were injected intraperitoneally sterile saline (SF) or endotoxin (LPS) on days 17-18 of pregnancy. Following birth, female pups were subdivided into two groups and injected either sterile saline (SF, n=17) or endotoxin (LPS, n=17) on postnatal day 60 and four experimental groups were formed (SF+SF, SF+LPS, LPS+SF and LPS+LPS). Blood samples were taken 4 hours after final injection. Plasma levels of IL-1β, TNF-α, corticosteron, LH, FSH and blood leucocyte ratios were evaluated.Results: Neutrophil % ratio was higher but lymphocyte % ratio was lower in SF+LPS, LPS+SF and LPS+LPS groups than SF+SF group. Corticosterone, LH and FSH levels were not different between the groups but TNF-α level of LPS+LPS groups was higher than SF+SF and LPS+SF groups. IL-1β level of SF+LPS group was higher than SF+SF and LPS+SF groups. Conclusion: The results suggest that prenatal and post-pubertal endotoxin exposure programs cytokine level neutrophil and lymphocyte percentages without affecting hypothalamo-pituitary-adrenal and –gonadal axes.

Keywords:

Prenatal endotoxin, postnatal endoxin, neutrophil, lymphocyte, IL-1 beta TNF-alpha, corticosterone, gonadotrophins,

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