Dilek MEYDAN, Semih TAN, Saim ÖZDAMAR, Hülya ÇETİN

MATERNAL BISPHENOL-A'NIN SIÇAN BÖBREK GELİŞİMİ ÜZERİNDEKİ ETKİSİNİN İNCELENMESİ

Bisphenol-A, bugün epoksi reçine üretiminde ve diğer non-polimer plastiklerde katkı maddesi olarak kullanılan östrojenik bir kimyasaldır. BPA'nın bugünkü yaygın kullanımı nedeniyle, insanların maruz kalması kaçınılmazdır. Bu maruziyet, çeşitli vücut sistemlerinde zararlı etkilere neden olmaktadır. Bu çalışmanın amacı, gebelik ve emzirme döneminde BPA'ya maruz kalan anne sıçanların yavrularının böbreklerinin gelişimi üzerindeki etkilerini araştırmaktır, çünkü yavrular plasenta ve süt yoluyla BPA'ya maruz kalmaktadır. Bu çalışmada, 13 yetişkin Wistar albino dişi sıçan 3 gruba ayrıldı. Grup 1'de (Kontrol grubu) sıçanlara sadece 1 ml/kg/gün mısır yağı intraperitoneal olarak uygulandı. Grup 2'deki (25 mg BPA grubu) sıçanlara 25 mg/kg/gün BPA; Grup 3'teki (50 mg grup) sıçanlara 5 hafta boyunca intraperitoneal olarak 50 mg/kg/gün BPA uygulandı. Deneyin sonunda, yavru sıçanların intrakardiyak kanı ve böbrek dokuları alındı ve üre, total protein, kreatinin, TAS, TOS, MDA değerleri incelendi. Çalışmanın sonunda, BPA'nın serum üre, kreatinin ve total protein seviyelerini artırdığı, böbrek dokusunda oksidatif hasara neden olan reaktif oksijen türlerinin oluşumunu indüklediği ve yavru sıçanların böbreklerinde, Bowman'ın boşluğunda daralma gibi ciddi yapısal hasarlara neden olduğu belirlendi. böbrek korpuskülü, proksimal, distal tübüller ve toplayıcı kanallarda genişleme, tübüler epitelde hücre kaybı ve vaküolizasyon.

Anahtar Kelimeler:

Bisfenol-A, üre, kreatinin, oksifatif stress

Examining the Impact of Maternally Administered Bisphenol-A on Rat Kidney Development

Aim: Bisphenol-A (BPA) is an estrogenic chemical used today in the production of epoxy resin and as an additive in other non-polymer plastics. Due to the widespread use of BPA today, human exposure is inevitable. This exposure causes harmful effects on various body systems. The aim of this study is to investigate the effects on the development of the kidneys of the offspring of mother rats exposed to BPA during pregnancy and lactation, as a result of the offspring being exposed to BPA through the placenta and milk. Material and Methods: In this study, 13 adult Wistar albino female rats were divided into 3 groups. In Group 1 (Control group), rats were only administered 1 ml/kg/day corn oil intraperitoneally. Group 2 (25 mg BPA group) rats were administered 25 mg/kg/day BPA; Group 3 (50 mg group) rats were administered 50 mg/kg/day BPA intraperitoneally for 5 weeks. At the end of the experiment, the intracardiac blood and kidney tissues of the offspring rats were taken and examined for urea, total protein, creatinine, TAS, TOS, MDA values. Results: At the end of the study, it was determined that BPA increased serum urea, creatinine and total protein levels, induced the formation of reactive oxygen species causing oxidative damage in kidney tissue, and caused serious structural damages Conclusion: Only mother rats exposed to BPA. BPA transferred to pups via placenta and milk, causing structural damage: narrowing in Bowman's space of renal corpuscle, dilatation in proximal/distal tubules and collecting ducts, occasional cell loss, vacuolization in tubule epithelia.

Keywords:

Bisphenol-A (BPA), urea, creatinine, oxidative stress,

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