Mustafa Yılmaz, Hilal Bektaş Uysal, Buket Demirci

Sıçanlarda Lipopolisakkarid ile İndüklenen Sistemik İnflamasyon Modelinde Folinik Asit (Lökovorin) Tedavisi

Amaç: Folinik asit [(FA); Lökovorin]’in septik şok modelinde antioksidan ve antiinflamatuvar etkisi araştırıldı. Gereç ve Yöntemler: FA (100 mg/kg), lipopolisakkarid (LPS; 5 mg/kg)’ten 30 dakika önce uygulandı. Kontrol, FA, LPS ve FA + LPS gruplarının kan ve doku örnekleri, LPS uygulamasından 16 saat sonra alındı. Bulgular: Sepsis, alanin aminotransferaz, aspartat aminotransferaz, üre, kreatinin, laktat, nitrik oksit, malondialdehitin (MDA), prokalsitonin, tümör nekroz faktörü, interlökin-1b düzeylerinde belirgin artışa ve dokularda antioksidanların [katalaz (CAT) ve glutatyon (GSH) düzeyleri] tüketilmesine yol açtı. FA tedavisi ile MDA, nitrik oksit, GSH ve CAT değerlerinde iyileşme sağlandı. Sonuç: FA, sepsiste böbrek ve karaciğer dokularında (anti)oksidan durumu onarmak için kullanılacak güvenli bir yoldur. İnflamatuvar mediyatörleri kontrolde, prokalsitonini azaltmada ve sepsisin metabolik sürecini düzenlemede etkili olabilir.

Folinic Acid (Leucovorin) Treatment in Lipopolysaccharide-Induced Systemic Inflammation in Rats

Objective: The antioxidant and anti-inflammatory effects of Folinic acid [(FA); Leucovorin] in a septic shock model have been investigated. Materials and Methods: FA (100 mg/kg) given thirty minutes before lipopolysaccharide (LPS; 5 mg/kg). The blood and tissue samples of control, FA, LPS and FA + LPS groups were obtained sixteen hours later of LPS intervention. Results: Sepsis resulted in a remarkable increase in alanine aminotransferase, aspartate aminotransferase, urea, creatinine, lactate, nitric oxide, Malondialdehyde (MDA), procalcitonin, tumour necrosis factor-alpha, Interleukin 1-beta levels and consumed antioxidants catalase (CAT) and glutathione (GSH) levels of the tissues. MDA, nitric oxide, GSH and CAT levels were ameliorated by FA treatment. Conclusion: FA can be used as a safe way of restoring the (anti)oxidant status of kidney and liver tissue in sepsis clinic. It can be effective in controlling inflammatory mediators, decreasing procalcitonin and regulating the metabolic process of sepsis.

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