Zerina HADZİC, Enes PASİC, Mirsada HUKİC, Mirjana Gojkov VUKELİC, Sanja HADZİC

Salivary Interleukin-6 Levels in Patients with Periodontitis Stage IV

Objective: The role of interleukin-6 (IL-6) in the pathogenesis of periodontal diseaseand tissue destruction at the periodontal site has been widely reported. This studyaimed to evaluate the salivary IL-6 levels in patients with stage IV periodontitis.Materials and Methods: The study included 28 patients who were diagnosed withperiodontitis stage IV and the control group of 22 periodontally healthy patients. Allthe patients were systemically healthy. Saliva samples were collected, and clinicalperiodontal measurements, including probing depth (PD), clinical attachment level(CAL), papilla bleeding index (PBI), the percentage of sites with bleeding on probing(BOP) %, plaque index (PI) and calculus index (CI), were recorded. The unstimulatedsaliva of each patient was collected by a saliva collector, and all samples wereanalysed using the enzyme-linked immunosorbent assay method for the detectionof IL-6.Results: The mean value of salivary IL-6 in patients with periodontitis stage IV was22.18±5.96 pg/mL. In the control group, the average measured value of IL-6 was2.23±2.17 pg/mL. The periodontitis group had a significantly higher salivary IL-6levels than the control group. A strong positive correlation was observed betweenthe salivary IL-6 and clinical periodontal parameters (PD, CAL, PBI, BOP %, PI andCI) in patients with periodontitis stage IV (p

Evre IV Periodontitis Hastalarında Tükürük İnterlökin-6 Seviyeleri

Amaç: İnterlökin-6’nın (IL-6) periodontal hastalık patogenezi ve periodontal doku yıkımındaki rolü geniş çapta ele alınmıştır. Çalışmanın amacı, evre IV periodontitis hastalarının tükürüklerindeki IL-6 seviyelerini değerlendirmektir. Gereç ve Yöntemler: Araştırmaya evre IV periodontitis teşhisi konulan 28 hasta ile 22 hastadan oluşan kontrol grubu dahil edildi. Hastaların tümü sistemik olarak sağlıklıydı. Tükürük örnekleri toplandı ve sondalama derinliği (SD), klinik ataşman seviyesi (KAS), papil kanama indeksi (PKİ), sondalamada kanama yüzdesi (SK %), plak indeksi (Pİ) ve diştaşı indeksini (Dİ) içeren klinik periodontal ölçümler kaydedildi. Her bir hastanın uyarılmamış tükürükleri, tükürük toplayıcı ile toplandı ve örneklerdeki IL-6 seviyesi enzim bağlı immünosorbent testi ile analiz edildi. Bulgular: Evre IV periodontitis hastalarının ortalama IL-6 değeri 22,18±5,96 pg/mL idi. Kontrol grubunun ortalama IL-6 değeri ise 2,23±2,17 pg/mL idi. Periodontitisgrubunun tükürük IL-6 seviyesi kontrol grubundan daha yüksek bulundu. Evre IV periodontitis hastalarının tükürük IL-6 seviyeleri ile klinik periodontal parametreleri (SD, KAS, PKI, SK %, PI ve DI) arasında anlamlı ve güçlü pozitif korelasyonlar gözlendi (p

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1. Chiappelli F, Iribarren FJ, Prolo P. Salivary biomarkers in psychobiological medicine. Bioinformation 2006; 1: 331-4.

2. Nagler RM, Hershkovich O, Lischinsky S, Diamond E, Reznick AZ. Saliva analysis in the clinical setting: revisiting an underused diagnostic tool. J Investig Med 2002; 50: 214-25.

3. Matthews DC. Periodontal medicine: a new paradigm. J Can Dent Assoc 2000 ; 66: 488-91.

4. Kc S, Wang XZ, Gallagher JE. Diagnostic sensitivity and specificity of host-derived salivary biomarkers in periodontal disease amongst adults: Systematic review. J Clin Periodontol 2020; 47: 289-308.

5. Hu S, Loo JA, Wong DT. Human saliva proteome analysis and disease biomarker discovery. Expert Rev Proteomics 2007; 4: 531-8.

6. Miller CS, Foley JD, Bailey AL, Campell CL, Humphries RL, Christodoulides N, et al. Current developments in salivary diagnostics. Biomark Med 2010; 4: 171-89.

7. Azuma MM, Samuel RO, Gomes-Filho JE, Dezan-Junior E, Cintra LT. The role of IL-6 on apical periodontitis: a systematic review. Int Endod J 2014; 47: 615-21.

8. Ramseier CA, Kinney JS, Herr AE, Braun T, Sugai JV, Shelburne CA, et al. Identification of pathogen and host-response markers correlated with periodontal disease. J Periodontol 2009; 80: 436-46.

9. Ebersole JL, Schuster JL, Stevens J, Dawson D 3rd, Kryscio RJ, Lin Y, et al. Patterns of salivary analytes provide diagnostic capacity for distinguishing chronic adult periodontitis from health. J Clin Immunol 2013; 33: 271-9.

10. Nanakaly HT. Interleukine-6 Level in Saliva of Patients with Chronic Periodontitis: A Case-Control Study. J Baghdad Coll Dent 2016; 28: 103-8.

11. Nagarajan R, Miller CS, Dawson D 3rd, Al-Sabbagh M, Ebersole JL. Patient-Specific Variations in Biomarkers across Gingivitis and Periodontitis. PLoS One 2015; 10: 0136792.

12. Papapanou PN, Sanz M, Buduneli N, Dietrich T, Feres M, Fine DH, et al. Periodontitis: Consensus report of workgroup 2 of the 2017 World Workshop on the Classification of Periodontal and Peri-Implant Diseases and Conditions. J Periodontol 2018; 89: 173-82.

13. Yue Y, Liu Q, Xu C, Loo WT, Wang M, Wen G, et al. Comparative evaluation of cytokines in gingival crevicular fluid and saliva of patients with aggressive periodontitis. Int J Biol Markers 2013; 28: 108-12.

14. SILNESS J, LOE H. PERIODONTAL DISEASE IN PREGNANCY. II. CORRELATION BETWEEN ORAL HYGIENE AND PERIODONTAL CONDTION. Acta Odontol Scand 1964; 22: 121-35.

15. Saxer UP, Mühlemann HR. Motivation und Aufklärung [Motivation and education]. SSO Schweiz Monatsschr Zahnheilkd 1975; 85: 905-19.

16. Hamp SE, Nyman S, Lindhe J. Periodontal treatment of multirooted teeth. Results after 5 years. J Clin Periodontol 1975; 2: 126-35.

17. Navazesh M, Kumar SK; University of Southern California School of Dentistry. Measuring salivary flow: challenges and opportunities. J Am Dent Assoc 2008; 139: 35-40.

18. Becerik S, Öztürk VÖ, Atmaca H, Atilla G, Emingil G. Gingival crevicular fluid and plasma acute-phase cytokine levels in different periodontal diseases. J Periodontol 2012; 83: 1304-13.

19. Branco-de-Almeida LS, Cruz-Almeida Y, Gonzalez-Marrero Y, Huang H, Aukhil I, Harrison P, et al. Local and Plasma Biomarker Profiles in Localized Aggressive Periodontitis. JDR Clin Trans Res 2017; 2: 258-68.

20. Ebersole JL, Nagarajan R, Akers D, Miller CS. Targeted salivary biomarkers for discrimination of periodontal health and disease(s). Front Cell Infect Microbiol 2015; 5: 62.

21. Costa PP, Trevisan GL, Macedo GO, Palioto DB, Souza SL, Grisi MF, et al. Salivary interleukin-6, matrix metalloproteinase-8, and osteoprotegerin in patients with periodontitis and diabetes. J Periodontol 2010; 81: 384-91.

22. Geng W, Ying Hui T, Sheng Gen S. Expression of IL-6 and TNF-α activities in saliva of chronic periodontitis patients. Chin J Conserv Dent 2009; 5: 11.

23. Teles RP, Likhari V, Socransky SS, Haffajee AD. Salivary cytokine levels in subjects with chronic periodontitis and in periodontally healthy individuals: a cross-sectional study. J Periodontal Res 2009; 44: 411-7.

24. Javed F, Ahmed HB, Saeed A, Mehmood A, Bain C. Whole salivary interleukin-6 and matrix metalloproteinase-8 levels in patients with chronic periodontitis with and without prediabetes. J Periodontol 2014; 85: 130-5.

25. Ng PY, Donley M, Hausmann E, Hutson AD, Rossomando EF, Scannapieco FA. Candidate salivary biomarkers associated with alveolar bone loss: cross-sectional and in vitro studies. FEMS Immunol Med Microbiol 2007; 49: 252-60.

26. Kurihara N, Bertolini D, Suda T, Akiyama Y, Roodman GD. IL-6 stimulates osteoclast-like multinucleated cell formation in long term human marrow cultures by inducing IL-1 release. J Immunol 1990; 144: 4226-30.

27. Noh MK, Jung M, Kim SH, Lee SR, Park KH, Kim DH, et al. Assessment of IL-6, IL-8 and TNF-α levels in the gingival tissue of patients with periodontitis. Exp Ther Med 2013; 6: 847-51.

28. Tatakis DN, Kumar PS. Etiology and pathogenesis of periodontal diseases. Dent Clin North Am 2005; 49: 491-516.