NESLİHAN ÇELİK, MERVE İŞCAN YAPAR, Ali TAGHİZADEHGHALEHJOUGHİ

Oxidative Alteration in Gingival Fibroblast Cells Induced By Bulk-Fill and Conventional Flowable Composites

Objective: The release of components from dental materials may cause oxidative stress which is crucial factor for tissue damage and cell apoptosis or death. The aim of this study was to evaluate the cytotoxicity of different flowable composites and this materials effect on total antioxidant capacity (TAC) and total oxidant status (TOS) level in human gingival fibroblast cell culture. Materials and Methods: Gingival fibroblast cells obtained from healthy persons were used for evaluation the cytotoxicity and oxidant status. Six flowable composites used were: two bulk-fill flowable composites (SureFil SDR, X-tra base), a self-adhering flowable composite (Vertise Flow), a highly filled flowable composite (GrandioSO Flow), two conventional flowable composites (Filtek Ultimate, Clearfil Majesty). Specimens in 3 mm diameter, 2 mm height were prepared from each composite (n=6) and were transferred to 24 well plates. Wells without composite material were used as the control group. After 24 h incubation period, cytotoxicity was determined by using the 3-(4,5 dimetylthiazol-2-yl)-2,5 diphenlytetrazolium bromide (MTT) assay. Oxidative alterations were assessed using TAC and TOS assay kits. Data were analyzed using the ANOVA and least significant differences posthoc test. Results: Cytotoxicity of six materials was significantly different from the control group (p

Bulk-Fill ve Geleneksel Akıcı Kompozitlerle Uyarılan Dişeti Fibroblast Hücrelerindeki Oksidatif Değişim

Amaç: Dental materyallerden salınan komponentler doku hasarı ve hücre apoptosisi veya ölümünde önemli bir faktör olan oksidatif stres oluşumuna sebep olabilir. Bu çalışmanın amacı; farklı akıcı kompozit rezinlerin sitotoksisitesini ve bu materyallerin insan dişeti fibroblast hücre kültüründe total antioksidan kapasite (TAK) ve total oksidan durum (TOD) seviyesine etkisini değerlendirmektir. Gereç ve Yöntemler: Sağlıklı bireylerden elde edilen diş eti fibroblast hücreleri sitotoksisite ve oksidan durum değerlendirmesi için kullanıldı. Bulk-fill akıcı kompozit (SureFil SDR, X-tra base), kendiliğinden bağlanan akıcı kompozit (Vertise Flow), yüksek dolduruculu akıcı kompozit (GrandioSO Flow), geleneksel akıcı kompozit (Filtek Ultimate, Clearfil Majesty) olmak üzere altı farklı materyal kullanılarak 3 mm çapında 2 mm yüksekliğinde örnekler hazırlandı (n=6) ve 24’lük doku kültür kabına yerleştirildi. Örnek yerleştirilmeyen kuyucuklar kontrol grubu olarak belirlendi. Yirmi dört saat boyunca inkübe edildikten sonra 3-(4,5 dimetylthiazol-2-yl)-2,5 diphenlytetrazolium bromide (MTT) analizi ile materyallerin sitotoksisitesi değerlendirildi. Oksidatif değişim TAK ve TOD analiz kitleri ile değerlendirildi. Elde edilen verilerin analizinde ANOVA ve en önemsiz farkla post-hoc testi kullanıldı. Bulgular: Uygulanan altı materyalin sitotoksisitesi kontrol grubu ile anlamlı farklılık gösterdi (p

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