Antibacterial Efficiency of Different Irrigation Solutions, Lasers and Photodynamic Therapy with Indocyanine Green in Root Canals Infected By Enterococcus Faecalis

Objective: The aim of this study was to evaluate bacterial reduction after using different irrigants, lasers and photodynamic therapy (PDT) in root canals infected by Enterococcus faecalis . Materials and Methods: Seventy human maxillary incisors were used in this study. After endodontic preparation, sixty teeth were infected by 20 µl E. faecalis (ATCC 29212) and specimens were incubated for 72 hours. Groups were formed according to the irrigation protocol used: group 1: negative control, group 2: positive control, group 3: 10 mL 2% chlorhexidine (CHX), group 4: 10 mL 5% NaOCl, group 5: diode laser, group 6: Er:YAG laser, and group 7: indocyanine green (ICG)-diode laser (PDT). After disinfection procedures the samples isolated from root canals were placed into blood agar, incubated at 37 °C for 24 h and then were counted for colony-forming units. One-Way ANOVA was used for the statistical evaluation of data. For multiple comparisons, Tamhane’s T2 test was used. Results: All investigated disinfection procedures had a significant reduction in the bacterial population (p0.05) and statistically more effective than both laser groups (p

Enterococcus Faecalis ile Enfekte Edilmiş Kök Kanallarında Farklı İrrigasyon Solüsyonlarının, Lazerlerin ve İndosiyanin Yeşili ile Fotodinamik Terapinin Antibakteriyel Etkinliği

Amaç: Bu çalışmanın amacı, Enterococcus faecalis ile enfekte edilmiş kök kanallarında farklı irrigasyon solüsyonları, lazerler ve fotodinamik tedavi (FDT) kullandıktan sonra bakteri sayısındaki azalmayı değerlendirmektir. Gereç ve Yöntemler: Bu çalışmada yetmiş adet insan üst çene kesici dişi kullanıldı. Kök kanal preparasyonundan sonra altmış diş 20 μL E. faecalis (ATCC 29212) ile enfekte edildi ve numuneler 72 saat inkübasyona bırakıldı. Gruplar, kullanılan dezenfeksiyon protokolüne göre oluşturuldu: grup 1: Negatif kontrol, grup 2: Pozitif kontrol, grup 3: 10 mL %2 klorheksidin (KH), grup 4: 10 mL %5 NaOCl, grup 5: Diyot lazer, grup 6: Er:YAG lazer ve grup 7: İndosiyanin yeşili (İSY)-diyot lazer (FDT). Dezenfeksiyon prosedürlerinden sonra kök kanallarından izole edilen numuneler kanlı agara yerleştirildi, 37 °C’de 24 saat inkübe edildi ve daha sonra koloni oluşturan birimler sayıldı. Verilerin istatistiksel değerlendirilmesi için tek yönlü varyans analizi, çoklu karşılaştırmalar için Tamhane’nin T2 testi kullanıldı. Bulgular: İncelenen tüm dezenfeksiyon prosedürleri bakteri popülasyonunda belirgin bir azalmaya neden olmuştur (p0,05), her iki lazer grubundan ise istatistiksel olarak anlamlı seviyede daha etkindi (p

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Meandros Medical And Dental Journal-Cover
  • ISSN: 2149-9063
  • Başlangıç: 2000
  • Yayıncı: Erkan Mor
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