Total Functional Sperm Fraction (TFSF) measurements were used to estimate the overall ability of males to achieve conception in 29 men from a group of infertile couples. TFSF is a more comprehensive criterion than the traditional semen measurements because it incorporates all important seminal parameters such as total cell count per ejaculate (TCE). percentages of normal morphology (N) and motility (M). and a measurement of progressive. straight-swimming spermatozoa (P) on a scale of 1-3. The application of sperm washing followed by swim-up procedure can improve TFSF while separating the motile, highly progressive spermatozoa from round cells, leucocytes, spermatids, contaminating microorganisms and anti-sperm antibodies. In a clinical trial of direct intraperitoneal insemination (DIPI), TFSF measurements were used for the evaluation of male fertility potential after sperm purification. TFSF measurements were utilized to standardize the number of active, highly progressive and morphologically normal spermatozoa for each individual case in every cycle of DIPI treatment Results of the clinical trial showed that two out of six couples participating in DIPI were successful in achieving conception and having healthy babies.