Since October 1989 at Istanbul University, Istanbul School of Medicine, Our Children-Leukemia Foundation and Department of Pediatric Hematology/Oncology we have focused on the systematic use of apheresis techniques especially for thrombocytopheresis. Fifty-six thrombocytopheresis were performed. Complete blood count, blood groups were determined and compatible donors with a platelet count > 200 000/p.L and negative serologic tests (Hepatitis B, CMV, HIV, malaria, syphilis) were selected. In 6/56 thrombocytopheresis (10.7%) the aimed number of cycles could not be achieved. Fifty applications were completed. Six units of platelets each from 29 donors (51.7%) and less than 6 units each from the other donors were collected. The platelet counts in the collected platelet concentrates were between 299- 1024x109/L. Each unit contained about 30-60 ml of platelet suspension. Each unit only contained very little amounts of RBC and WBC contamination. Apheresis techniques based on autosurge automated protocols are very useful and needed in every center applying intensified chemotherapy protocols which need vigorous supportive measures.