Ergene Nehir Suyunun Soğan (Allium cepa L.) Kök Ucu Hücrelerinde Genotoksik Etkisinin Değerlendirilmesi
Türkiye'de endüstriyel hareketlerin artması ile birlikte, Trakya bölgesinde organize sanayi alanları kurulmuş ve bunun sonucunda özellikle Trakya bölgesini besleyen Ergene Nehri ve çevre alanlarında kirlilik artmıştır. Bu çalışmada, Ergene nehir suyunun Allium cepa L. kök ucu hücrelerinde genotoksik ve sitotoksik etkilerinin araştırılması amaçlanmıştır. Çalışmada Ergene nehir suyundan iki farklı istasyondan aynı dönemde alınan su örnekleri 0.1/100 mL, 1/100 mL, 5/100 mL, 10/100 mL, 20/100 mL ve 40/100 mL konsantrasyonlar ile soğan (Allium cepa L.) kök ucu hücreleri 24 saat muamele edilerek sitogenetik anormallikler belirlenmiş ve mitotik indeks hesaplanmıştır. Her iki istasyondan alınan örneklerin, uygulanan tüm dozlarında mitotik anormallikleri kontrole göre anlamlı bir şekilde arttırdığı ve mitotik indeksi anlamlı olarak değiştirdiği belirlenmiştir. En fazla gözlenen mitotik anormallikler kromozomlarda asenkronizasyon, yanlış kutuplaşma ve C-mitozdur. Sonuçlar Ergene nehir suyunun A. cepa testinde genotoksik ve sitotoksik etkili olduğunu göstermektedir
Evaluation Of Genotoxic Effects On Onion (Allium cepa L.) Root Tip Cell Of Ergene River Water
As a result of industrialization movement in Turkey, organized industrial zone have been established in Thrace, and especially pollution reached significant size in there and their surrounding area. In the present study, it was aimed to study the genotoxic and cytotoxic effects of the Ergene River water on Allium cepa root tip cells. In the study, Ergene river water samples taken at the same time from two different stations. Samples were exposed with 0.1/100 mL, 1/100 mL, 5/100 mL, 10/100 mL, 20/100 mL, and 40/100 mL doses for 24 hours on onion (Allium cepa L.) root tip cells, and cytogenetic abnormalities and mitotoic index were investigated. On applied all doses of both stations, mitotic abnormalities were significantly increased compared with control, and mitotic index was significantly changed. The most observed mitotic abnormalities in chromosomes are asencronization, incorrect polarization, and C-mitosis. The results indicated that the Ergene River has genotoxic, and cytotoxic effects on A. cepa
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- ISSN: 2146-5150
- Yayın Aralığı: Yılda 4 Sayı
- Başlangıç: 2011
- Yayıncı: Marmara Üniversitesi Fen Bilimleri Enstitüsü