Extracts and preparations made from the animal origin were used extensively in folk and modern medicine for treating many human diseases. The objective of this study was to determine the antimicrobial and antifungal activities of aqueous skin mucus from rainbow trout. Evaluation of inhibition effect of the aqueous skin mucus against Staphylococcus aureus-ATCC 6538, Escherichia coli-ATCC 9837, Citrobacter freundii-ATCC 8090, Enterobacter aerogenes-ATCC 13048, Neisseria lactamica-ATCC 23970, Proteus vulgaris-ATCC 49990, Proteus mirabilis-ATCC 29906, Pseudomonas aeruginosa-ATCC 9027, Pseudomonas fluorescens-ATCC 13525, Bacillus cereus-ATCC 10987, Klebsiella pneumonia-ATCC 13883, Klebsiella oxyttoca-ATCC 43863, Streptococcus pneumoniae-ATCC 49619, Candida albicans-ATCC 90028 and Candida parapsilosis-ATCC 22019 was tested. The antimicrobial and antifungal effects of mucus on the bacteria were assessed by a disc diffusion assay. The activity was measured in terms of zone of inhibition in mm. The mucus did not show antibacterial and antifungal activities against any of the tested strains of bacteria and fungi yeast.