The antagonistic activity of Pseudomonas bacteria against the phytopathogen fungi of agricultural plants was studied. The laboratory collection of Pseudomonas bacteria has been tested for a biological activity to different species of phytopathogen fungi. P.fluorescens, 7-ISS and P.fluorescens, KD -5 -3 strains have shown a high effecitive and broad spectrum antibiotic activity to phytopathogen fungi of agricultural plants