Ali GÖÇER, Mehmet ALMA, Yekta GEZGİNÇ, Eyyüp KARAOĞUL

The Effect of Some Parameters on the Production of L(+) Lactic Acid Using Wheat Wastewater by Rhizopus oryzae NRRL-395

The aim of the study was to investigate some major parameters onproduction lactic acid using wheat wastewater by Rhizopus oryzaeNRRL-395. The parameters, which may play an effective role inproduction, were determined. For developing and producing pure L(+)-Lactic acid of the filamentous fungus Rhizopus oryzae NRRL-395, richmedium with wheat wastewater and glucose as carbon source wereused in the study. The effects of carbon sources concentration, pH,agitation (shaking speed) and spores concentration on lactic acidproduction were examined. After fermentation process, the highestvalues of lactic acid obtained from wheat wastewater and glucose wereanalyzed using high-performance liquid chromatography withultraviolet detectors (HPLC-UV). The maximum L (+) Lactic acidproduction in shake flasks was investigated at pH 6, 1.0x106spores/mL, 150 rpm, 30 ̊C at 8 days. The maximum lactic acid contentfor wheat wastewater and glucose were obtained at a concentration of100% (5.638 g/L) and 150 g/L (5.042 g/L). The maximum lactic acidamount of 5.568 g/L was obtained at pH value of 6 for wheatwastewater. However, the maximum lactic acid amount of 2.463 g/Lwas obtained at pH value of 6 for glucose. The maximum lactic acidvalues for wheat wastewater and glucose were obtained 5.603 g/L and2.483 g/L at 150 rpm speed, respectively. The maximum lactic acidvalues for wheat wastewater and glucose were obtained 5.804 g/L and5.646 g/L at the 1.0x106 spores/mL respectively.

Atık Bulgur Suyu Kullanılarak Rhizopus oryzae NRRL-395 ile Üretilen Laktik Asite Bazı Parametrelerin Etkisi

Bu çalışmanın amacı, Rhizopus oryzae NRRL-395 tarafından bulgur atık suyu kullanılarak üretilen laktik asit üretimine bazı önemli parametrelerin etkisi denenmiştir. Bu amaç ile filamentli bir yapıya sahip olan Rhizopus oryzae fungusunun gelişimi ve laktik asit üretimi için bulgur atık suyu ve glukoz gibi karbon kaynakları bu çalışmada kullanılmıştır. Karbon kaynaklarının konsantrasyonunun, pH’larının, çalkalama hızlarının ve spor konsantrasyonlarının üretim üzerine olan etkileri incelenmiştir. Fermentasyon işleminden sonra, bulgur atık suyu ve glukozdan elde edilen yüksek laktik asit değerleri yüksek performanslı sıvı kromatografisi (HPLC-UV) kullanılarak analiz edilmiştir. Çalkalamalı sistemde en yüksek laktik asit değerleri için pH 6, 1.0x106 spor/mL konsantrasyonunda, 150 rpm hızında ve 30 ̊C de 8 gün olarak araştırılmıştır. Bulgur atık suyu için maksimum laktik asit miktarı %100 konsantrasyonda (5.638 g/L) ve glukoz için 150 g/L konsantrasyonda (5.042 g/L) olarak elde edilmiştir. Bulgur atık suyu ve glukoz için maksimum laktik asit oranları sırası ile pH 6’da 5.568 ve 2.463 g/L, 150 rpm hızında 5.603 ve 2.483 g/L ve 1.0x106 spor/mL konsantrasyonunda ise 5.804 ve 5.646 g/L olarak elde edilmiştir.

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