Hilal ERDOĞAN, TUFAN CAN ULU, HAYRETTİN KUŞÇU

The Effect of Different Dripper Properties on Entomopathogenic Nematode Application in Drip Irrigation

There are many types of drippers with different flow path length, flowpath shape and filtration surface. EPN delivery performance of themost commonly used four different types of drippers (in-line shortpath, in-line long path, in-line cylindrical and on-line button) wasexamined with a drip irrigation system in laboratory conditions.Under four different pressures (0.5, 1, 1.5, 2 bar), EPNs were appliedto 1-liter beakers with irrigation system and discharged nematodeswere counted under a stereomicroscope. The effect of pressure onapplication and EPN mortality were also determined. The resultsshowed that there were significant differences between the dischargeratio of EPNs from drippers. Among the four drippers, on-line buttondripper sustained the highest and fastest discharge ratio. Pressurealone had no significant effect on delivering EPNs. However, it shouldbe considered that long pressure exposure may harm EPNs.Regarding our results, different irrigation drippers have significantlydifferent effects on EPN discharge ratio. Therefore, optimizing dripirrigation systems for EPN applications may increase theirperformance.

Damla Sulamada Farklı Damlatıcı Özelliklerinin Entomopatojen Nematod Uygulamasına Etkisi

Damla sulamada kullanılan damlatıcıların akış yolu uzunluğu, akış yolu şekli, damlatıcı debisi vb. farklı özellikleri bulunmaktadır. Bu çalışmada damla sulamada yaygın olarak kullanılan dört farklı damlatıcının (içten geçik kısa akış yollu damlatıcı, içten geçik uzun akış yollu damlatıcı, içten geçik silindir tipli damlatıcı, üstten geçik katif damlatıcı) EPN uygulamasındaki performansları laboratuvarda kurulan damla sulama sistemiyle karşılaştırılmıştır. Dört farklı basınç altında (0.5, 1, 1.5, 2 bar) EPN’ler 1 litrelik beherlere uygulanmış ve beherlerdeki EPN’ler süzülerek mikroskop altında sayılmıştır. Çalışmada basıncın ve uygulamaya ve EPN üzerinde ölümcül etkisi de incelenmiştir. Sonuçlar değerlendirildiğinde farklı damlatıcılardan çıkan EPN miktarları arasında önemli farklılıklar olduğu tespit edilmiştir. Denemelerde üstten geçik katif damlatıcı ile yapılan uygulamalarda en fazla ve en hızlı EPN çıkışı olduğu tespit edilmiştir. Basıncın tek başına, uygulamaya ve EPN üzerine herhangi bir etkisinin olmadığı tespit edilmesine rağmen uzun süreli yüksek basınca maruz kalan EPN’lerin zarar görebileceği göz önünde bulundurulmalıdır. Çalışma sonuçları incelendiğinde farklı damlatıcıların EPN uygulamasında farklı çıkış oranları gösterdiği belirlenmiştir. Bu nedenle gelecekte damla sulama sistemlerinin EPN uygulaması üzerine optimize edilmesi ile başarı şansının artacağı düşünülmektedir.

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Arrington AE, Kennedy GG, Abney MR 2016. Applying insecticides through drip irrigation to reduce wireworm (Coleoptera: Elateridae) feeding damage in sweet potato. Pest Management Science 72 (6): 1133–1140.

Conner JM, McSorley R, Stansly PA, Pitts DJ 1998. Delivery of Steinernema riobravis through a drip irrigation system. Nematropica 28 (1): 95–100.

Curran J 1992. Influence of application method and pest population size on the field efficacy of entomopathogenic nematodes. Journal of Nematology 24 (4S): 631–636.

Curran J, Patel V 1988. Use of A Trickle Irrigation System to Distribute Entomopathogenic Nematodes (Nematoda: Heterorhabditidae) for The Control Of Weevil Pests (Coleoptera: Curculionidae) Of Strawberries. Australian Journal of Experimental Agriculture 28 (5): 639– 643.

Ehlers R-U 2001. Mass production of entomopathogenic nematodes for plant protection. Applied Microbiology and Biotechnology 56 (5–6): 623–633.

Ehlers R-U 2005. Forum on safety and regulation (Nematodes as biocontrol agents, CABI: Wallingford, Eds. Grewal PS, Ehlers RU, ShapiroIlan DI) 107–114.

Ellsbury MM, Jackson JJ, Woodson WD, Beck DL, Stange KA 1996. Efficacy, application distribution, and concentration by stemflow of Steinernema carpocapsae (Rhabditida: Steinernematidae) suspensions applied with a lateral-move irrigation system for corn rootworm (Coleoptera: Chrysomelidae) control in maize. Journal of Economic Entomology 89 (1): 74–81.

Fife JP, Derksen RC, Ozkan HE, Grewal P 2003. Effects of pressure differentials on the viability and infectivity of entomopathogenic nematodes. Biological Control 27 (1): 65–72.

Gan-Mor S, Matthews GA 2003. Recent developments in sprayers for application of biopesticides - An overview. Biosystems Engineering 119–125.

Grewal PS 2002. Formulation and application technology (Entomopathogenic Nematology, CABI, Wallingford, Ed. Gaulger R) 265–287.

Johnigk S, Ecke F, Poehling M, Ehlers R-U 2004. Liquid culture mass production of biocontrol nematodes, Heterorhabditis bacteriophora (Nematoda: Rhabditida): improved timing of dauer juvenile inoculation. Applied microbiology and biotechnology 64 (5): 651–8.

Kaplan F, Alborn HT, von Reuss SH, Ajredini R, Ali JG, Akyazi , Stelinski, LL, Edison AS, Schroeder FC, Teal PE 2012. Interspecific nematode signals regulate dispersal behavior. PLoS ONE 7 (6): e38735.

Kaya HK, Gaugler R 1993. Entomopathogenic Nematodes. Annual Review of Entomology 38 (125): 181–206.

Kaya HK, Stock SP 1997. Techniques in insect nematology (Manual of Techniques in Insect Pathology, Elsevier, Ed. Lawrence A) 281–324.

Lacey LA, Grzywacz D, Shapiro-Ilan DI, Frutos R, Brownbridge M, Goettel MS 2015. Insect pathogens as biological control agents: Back to the future. Journal of Invertebrate Pathology 132: 1–41.

Nimkingrat P, Strauch O, Ehlers R-U 2013. Hybridisation and genetic selection for improving desiccation tolerance of the entomopathogenic nematode Steinernema feltiae. Biocontrol Science and Technology 23 (February 2014): 348–361.

Perret-Gentil A, Mirti A, Giurintano J, Sampson E, Gao X, Shapiro-Ilan D, Kaplan F 2017. Lack of pheromone reduces nematode dispersal (En annual meeting the american-phytopathological-society (aps), sanantonio, tx, aug 05-09, 2017). Phytopathology 107 (12, S): 110.

Peters A 1996. The natural host range of Steinernema and Heterorhabditis spp. and their impact on insect populations. Biocontrol Science and Technology 6 (3): 389–402.

Raja RK, Hazir C, Gümüş A, Asan C, Karagöz M, Hazir S 2015. Efficacy of the entomopathogenic nematode Heterorhabditis bacteriophora using different application methods in the presence or absence of a natural enemy. Turkish Journal of Agriculture and Forestry 39 (2): 277–285.

Reed D, Reed G, Creighton C 1986. Introduction of entomogenous nematodes into trickle irrigation systems to control striped cucumber beetle, (Coleoptera: Chrysomelidae). Journal of Economic Entomology 79: 1330–1333.

Salame L, Glazer I, Chubinishvilli MT, Chkhubianishvili T 2010. Genetic improvement of the desiccation tolerance and host-seeking ability of the entomopathogenic nematode Steinernema feltiae. Phytoparasitica 38 (4): 359–368.

Sayinci B, Bastaban S 2008. The Role of Spray Units on Biological Control Agent Application. 39 (1): 151–157.

Segal D, Glazer I 2000. Genetics for improving biological control agents: the case of entomopathogenic nematodes. Crop Protection 19 (8–10): 685–689.

Shapiro-Ilan DI, Han R, Dolinksi C 2012. Entomopathogenic Nematode Production and Application Technology. Journal of nematology 44 (2): 206–17.

Stock SP, Hunt DJ 2005. Morphology and systematics of nematodes used in biocontrol (Nematodes as biocontrol agents, CABI, Wallingford, Eds. Grewal PS, Ehlers RU, Shapiro-Ilan DI) 3–43.

Susurluk IA, Ehlers R-U 2008. Field persistence of the entomopathogenic nematode Heterorhabditis bacteriophora in different crops. BioControl 53 (4): 627–641.

Ulu TC, Susurluk IA 2014. Heat and desiccation tolerances of Heterorhabditis bacteriophora strains and relationships between their tolerances and some bioecological characteristics. Invertebrate Survival Journal 11: 4–10.

Wang X, Zhu H, Reding ME, Locke JC, Leland JE, Derksen RC, Spongberg AL, Krause CR 2009. Delivery of chemical and microbial pesticides through drip irrigation systems. Applied Engineering in Agriculture 25 (6): 883–893.

Wennemann L, Cone WW, Wright LC, Perez, Conant MM 2003. Distribution patterns of entomopathogenic nematodes applied through drip irrigation systems. Journal of Economic Entomology, 96, 287-291. 96 (2): 287–291.

Wright DJ, Peters A, Schroer , Fife JP 2005. Application technology (Nematodes as biocontrol agents, CABI, Wallington, Eds. Grewal PS, Ehlers RU, Shapiro-Ilan DI) 91–106.

Wright R, Witkowski J, Echtenkamp G, Georgis R 1993. Efficacy and Persistence of Steinernema carpocapsae (Rhabditida: Steinemematidae) Applied through a Center-Pivot Irrigation System Against Larval Corn Rootworms (Coleoptera: Chrysomelidae). Journal of Economic Entomology 86 (5): 1348–1354.