Meşe Palamudunun (Quercus coccifera L.) Farklı Kısımlarının A549, MCF-7 ve HeLa İnsan Kanser Hücrelerine Karşı Antikanser, Antiproliferatif ve Laktat Dehidrojenaz Enzim Aktiviteleri

Bu çalışmada, Meşe palamudunun (Q. coccifera) dış-kabuk, külah veiç kısmından elde edilen özütlerin; A549, MCF-7, HeLa kanserhücreleri ve kanserli olmayan HUVEC hücrelerine karşı potansiyelantikanser, antiproliferatif ve nekrotik kapasiteleri araştırılmıştır.Analizler sonucunda, meşe palamudunun farklı kısımlarından eldeedilen ekstrelerin en düşük konsantrasyonda dahi antikanser veantiproliferatif etkiye sahip oldukları ortaya konulmuştur. Palamutmeyvesinin külah kısmından elde edilen etanol özütleri, 5.04±0.02 ile18.04±0.16 µg/mL arasında değişen IC50 değerleriyle, diğerkısımlarından elde edilen özütlerden daha yüksek antikanser aktivitegöstermiştir. En yüksek oranda sitotoksik aktivite MCF-7 hücrelerinekarşı gözlenirken; en düşük sitotoksik aktivite ise A549 hücrelerindegözlenmiştir. Antikanser ve antiproliferatif aktivite sonuçlarınınaksine, en yüksek LDH aktivitesi HeLa hücrelerinde belirlenmiştir.MTT ve tripan mavisi analiz sonuçlarıyla uyumlu olarak, en yüksekLDH salınım kapasitesi külah kısmından elde etanol özütlerindesaptanmıştır. Bu çalışma; meşe palamudunun, kanser hücrelerindehücre büyümesini konsantrasyon ve zamana bağlı olarak engellemepotansiyeline sahip olduğunu ortaya koyan, özgün bir çalışmadır.

Anticancer, Antiproliferative and Lactate Dehydrogenase Enzyme Activities of Different Parts of Acorn (Quercus coccifera L.) against A549, MCF-7 and HeLa Human Cancer Cells

In this study, potential anticancer antiproliferative and necrotic capacities of the extracts obtained from shell, cup and shelled acorn parts of acorn (Q. coccifera) were investigated against A549, MCF-7, HeLa cancer cells and non-tumorous HUVECs. As a result of the analysis, it was proved that the different parts obtained from the acorns have anticancer and antiproliferative effects, even at the lowest concentration. The ethanol extracts obtained from the cup parts of acorn showed higher anticancer activity with IC50 values ranging from 5.04±0.02 to 18.04±0.16 µg/mL than the extracts from other parts. The highest rate of cytotoxic activity was observed against MCF-7 cells, whilst the lowest cytotoxic activity was observed against A549 cells. In contrast to anticancer and antiproliferative activity results, the highest LDH activity was determined in HeLa cells. In accordance with the MTT and trypan blue assay results, the highest LDH release capacity was found in the ethanol extracts obtained from the cup part. This is an original study that reveals acorn has the potential to inhibit cell growth in the cancer cells depending on concentration and time.

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