Lotus maritimus L.'nin (Leguminosae) in Vitro Rejenerasyon Potansiyeli

Lotus maritimus L.'nin rejenerasyon potansiyelini araştırmak için, L. maritimus'un tohumları in vitro koşullarda ekilmiş ve daha sonra kök, hipokotil, kotiledon, sap ve epikotil parçaları, farklı αnaphthalene acetic acid (NAA) konsantrasyonları (1, 2 ve 4 mg L-1) ve sitokinin tiplerinin [0 (kontrol), 1 mg L-1 6-Benzilaminopurin (BAP) ve 0.5 mg L-1 kinetin] kombinasyonlarını içeren MS ortamlarında kültüre alınmıştır. Sonuçlara göre, en yüksek kallus indüksiyonu, sap ve epikotil eksplantlarından 1 veya 2 mg L-1 NAA konsantrasyonu + 0.5 mg L-1 kinetin ile sağlanmıştır. En iyi bitki rejenerasyonu değerleri (3.56 ve 3.20 sürgün/kallus) ise epikotil ve hipokotil parçalarının 1 mg L-1 NAA içeren MS ortamında kültüre alınmasıyla elde edilmiştir. Oluşan sürgünler, 1 mg L-1 NAA içeren ½ MS ortamında kolayca köklendirilmiş ve büyük ölçüde dış ortama aktarılmıştır.

In Vitro Regeneration Potential of Lotus maritimus L. (Leguminosae)

To investigate the regeneration potential of Lotus maritimus L., thestem, hypocotyl, cotyledon, root, and epicotyl were cultured in MSmedia containing different combinations of α-naphthalene acetic acid(NAA) concentrations (1, 2, and 4 mg L-1) and cytokinin types [0(control), 1 mg L-1 of 6-Benzilaminopurin (BAP), and 0.5 mg L-1 ofkinetin]. The highest callus induction was achieved with the stem andepicotyl explants at 1 or 2 mg L-1 concentrations of NAA plus 0.5 mgL-1 of kinetin. The best plant regeneration values (3.56 and 3.20 shootsper callus) were determined with the epicotyl and hypocotyl culturedin MS media containing 1 mg L-1 of NAA. The obtained shoots wereeasily rooted in half-strength MS media containing 1 mg L-1 NAA andsubstantially adapted to the external environment.

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