GAMZE YILDIZ, Ceren AKTÜRK, Merve ÖZERKAN, ÖZER YILMAZ

Linum arboreum L. (Linaceae) Türünün Antioksidan İçeriği ve Serbest Radikal Süpürücü Aktivitesi

Günümüzde, yapılan çalışmalar bitkilerin karotenoidler, pigmentler,fenoller ve flavonoidler gibi antioksidanlar bakımından zenginolduğunu göstermektedir. Bu nedenle çalışma Linum arboreum L.yaprak ve meyve metanolik özütlerinin serbest radikal süpürücüaktivitesini ve antioksidan içeriğini belirlemek için tasarlanmıştır.Bu amaçla çalışmada L. arboreum türünün 2,2-diphenyl-1-picrylhydrazyl (DPPH) radikal süpürme aktivitesi, 2,2’-azinobis (3-etil-bezotiazolin 6 sulfonat) (ABTS) radikal süpürme aktivitesi,indirgeme gücü, toplam antioksidan, toplam fenol ve toplamflavonoid içerikleri belirlenmiştir. Her iki özütün antioksidanetkinliğini değerlendirmek için DPPH ve ABTS testlerinde IC50değerleri hesaplanmıştır. Sonuçlarımıza göre sırasıyla yaprak vemeyve için IC50 değerleri DPPH analizinde 106.55  1.20 g ml-1 ve108.56  2.65 g ml-1 iken, ABTS analizinde 1144.8  38.44 g ml-1 ve1114.7  37.09 g ml-1 olarak hesaplanmıştır. Yaprak ve meyveözütlerinin serbest radikal temizleme aktiviteleri istatistiksel olarakbirbirinden farklı bulunmamıştır. Bununla birlikte meyve özütü ilekıyaslandığında, yaprak özütü önemli ölçüde daha yüksek toplamflavonoid ve toplam antioksidan içeriği göstermiştir. Sonuç olarakveriler L. arboreum türünün potansiyel bir doğal antioksidankaynağı olarak değerlendirilebileceğini göstermiştir.

Free Radical Scavenging Activity and Antioxidant Contents of Linum arboreum L. (Linaceae)

Nowadays, in studies show that plants are rich in antioxidant such as carotenoids, pigments, phenols and flavonoids. Therefore, the present study was designed to evaluate the free radical scavenging activity and antioxidant content of methanol extract of leaf and fruit of Linum arboreum L. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, 2,2’-azinobis (3-etil-bezotiazolin 6 sulfonat) (ABTS) radical scavenging activity, reducing power, total antioxidant, total phenolic and total flavonoid assay were carried out to evaluate the antioxidant potential of the methanolic extracts. The IC50 values were calculated for the DPPH and ABTS assays in order to evaluate the antioxidant efficiency of each of the L. arboreum extracts. According to our results, IC50 values for DPPH were 106.55  1.20 g ml-1 and 108.56  2.65 g ml-1 while IC50 values for ABTS were 1144.8  38.44 g ml-1 and 1114.7  37.09 g ml-1 for the leaf and fruit extracts, respectively. Free radical scavenging activity of leaf and fruit extracts did not differ significantly from each other. However, leaf extract showed a significantly greater total flavonoid and total antioxidant contents compared to fruit extract. As a result, L. arboreum could be considered as a potential source of natural antioxidant.

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