Kapsaisinin H2452 Mezotelyoma Hücre Hattından Seçilen Klonlarda Sitotoksisitesinin ve Kaspaz-3 Üzerine Etkilerinin Belirlenmesi

Mezotelyoma; kalp, akciğer ve karın gibi iç organların yüzeyinikaplayan mezotelyum dokusundan gelişen bir kanser türüdür vetümörlerinin poliKlon al kökene sahip olduğu düşünülmektedir.Kapsaisinin kanser hücreleri üzerindeki apoptotik etkileribilinmektedir. Bu çalışmada; H2452 mezoteliyomahücre hattındanseçilmiş dört (I, II, III ve IV numaralı) Klon üzerinde kapsaisininsitotoksisitesi ve kaspaz-3 ifadesine ve aktivasyonuna etkileri tespitedilmeye çalışılmıştır. Bu amaçla; öncelikle, Klon ların IC50 değerleriMTS [3-(4,5)-dimetiltiazol-2-il)-5-(3-karboksimetoksifenil)-2-(4-sülfofenil)-2H-tetrazolyum] tuzunun formazana indirgenmesimetoduyla belirlenmiş ve kapsaisin sitotoksisitesinin bazı Klon lararasında istatistiksel olarak anlamlı bir fark gösterdiği (p

Determination of the Cytotoxicity of Capsaicin and its Effects on Caspase-3 in the Clones Selected from H2452 Mesothelioma Cell Line

Mesothelioma is a type of cancer that develops from the mesothelium tissue that covers the surface of the internal organs such as the heart, lungs and abdomen, and its tumors are thought to have polyclonal origin. Apoptotic effects of capsaicin on cancer cells are known. In this study; we tried to determine the cytotoxicity of capsaicin and its effects on caspase-3 expression and activation on four (clone-I, -II, -III and - IV) clones selected from the H2452 mesothelioma cell line. For this purpose; firstly, capsaicin cytotoxicity, i.e. IC50 values of clones were determined by the method of reduction of MTS [3- (4,5) - dimethylthiazol-2-yl) -5- (3-carboxymethoxyphenyl) -2- (4- sulfophenyl) -2H-tetrazolium] salt to formazan. It has been demonstrated that the type of the clones was affecting the IC50’s of capsaicin statistically significantly (p

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