Yerleşim Sıklığının Sprague Dawley Ratlarda Büyüme Performansı, Bazı Serum Oksidatif ve Nitrosatif Stres Parametreleri Üzerine Etkisi

Bu çalışma, normal ve dar yerleşim sıklığında yetiştirilen dişi Sprague-Dawley ratların büyüme performanslarının ve bazı stres parametrelerinin karşılaştırılması amacıyla yapılmıştır. Denemede 4 haftalık yaştaki 72 adet Sprague Dawley cinsi dişi rat kullanılmıştır. Normal ve dar yerleşim sıklığı grupları için deneme başı canlı ağırlık ortalamaları 54.22±2.65 ve 55.53±2.66 g olarak hesaplanmıştır. Hayvanlar 1 ay boyunca haftalık olarak tartılmış ve ağırlıkları arttıkça hayvan başına düşen taban alanları tedrici olarak artırılmıştır. Dişi ratlar, 8-9. haftada cinsel olgunluğa eriştiği için, deneme hayvanlar 2 aylık oluncaya kadar sürdürülmüştür. Ratların haftalık tartım sonuçlarına göre canlı ağırlıkların her iki grup için düzenli bir artış gösterdiği ve büyüme performanslarının yerleşim sıklığına göre değişmediği tespit edilmiştir (P>0.05). Serum Toplam Antioksidan Kapasite (TAC) ve Glutatyon (GSH) değerlerinin yerleşim sıklığından etkilendiği ve dar yerleşim sıklığında yetiştirilen ratlarda önemli ölçüde azaldığı sonucuna varılmıştır. Toplam Oksidan Kapasite (TOC), Nitrik oksit (NO) ve Malondialdehit (MDA) değerlerinin ise dar yerleşim sıklığı grubundaki ratlarda yaklaşık 1.5 kat arttığı belirlenmiştir. Sonuç olarak artan stoklama yoğunluğunun hayvanların büyüme performansı üzerine olumsuz bir etkisi olmasa bile, hayvanları strese sokarak kan değerlerinde bir takım fizyolojik değişikliklere yol açtığı saptanmıştır. Bu sebeple bilimsel çalışmalarda en doğru sonucun elde edilebilmesi için hayvanların mutlaka ideal barındırma koşullarında yetiştirilmiş olmasına dikkat edilmelidir.

The Effects on the Growth Performance, Some Serum Oxidative and Nitrosative Stress Parameters of the Stocking Density in the Sprague-Dawley Rats

Objective of his studywas to compare the growth performances ofSprague-Dawley female rats fed at normal and narrowed stockingdensity with certain stress parameters. 72 Sprague-Dawley femalerats aged 4 weeks were used in the experiment. The average liveweight per trial for normal and narrow stock density groups wascalculated as 54.22 ± 2.65 and 55.53 ± 2.66 g. Animals’ weekly weighsduring a month indicated that as their weight increased, the floor areaper animal gradually expanded. Female rats reach sexual maturity at8-9 weeks. For this reason, the experiment was continued until theanimals were 2 months old. Based on the weekly weighing results ofthe rats, it was found that live weights increased regularly for bothgroups and growth performances did not change according to stockingdensity (P>0.05). It was concluded that serum Total AntioxidantCapacity (TAC) and Glutathione (GSH) values were affected by thestocking density and that significantly reduced in the rats fed atnarrowed stocking density. On the other hand, Total Oxidant Capacity(TOC), Nitric Oxide (NO) and malondialdehyde (MDA) valuesincreased approximately 1.5 times in the rats at the group of narrowedstocking density. As a result, it was found that even if the increasingstocking density has no negative effect on the growth performance ofthe animals, it causes some physiological changes in blood values bystressing the animals. Therefore, in order to get the most accurateresults in scientific studies, it must be paid attention to breed theanimals at the ideal housing conditions.

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