Nazım SEKEROGLU, Ceren Serin TANRIOVER, Sevgi GEZİCİ, Fatih YAYLA

İnsan Kanser Hücrelerinde Astragalus elongatus subsp. nucleiferus’ un Antikanser, Antiproliferatif ve Laktat Dehidrojenaz Enzim Aktiviteleri

Bu araştırmada, Astragallus elongatus subsp. nucleiferus’ un toprak üstü ve kök kısımlarından elde edilen etanol ve su özütlerinin potansiyel antikanser ve antiproliferatif aktivitelerinin araştırılması amaçlanmıştır. Bitki özütlerinin A549, H1299, C6 insan kanser hücreleri ve kanserli olmayan HUVEC hücrelerine karşı sitotoksisite ve hücre proliferasyon potansiyellerini araştırmak için; MTT, tripan mavisi ve LDH enzim aktivite analizleri yapılmıştır. MTT ve tripan mavisi deneylerinin bir sonucu olarak, test edilen insan akciğer ve beyin karsinoma hücrelerinde doza bağlı antikanser ve antiproliferatif etkiler gözlenmiştir. Bitkinin kökünden elde edilen su özütleri, 5.81±0.46 ile 18.24±0.12μg / mL arasında değişen IC50 değerleriyle, toprak üstü özütlerinden daha yüksek bir antikanser aktivite göstermiştir. Antikanser ve sitotoksisite sonuçlarının aksine, kök etanol özütleri doza bağlı olarak en yüksek apoptoz seviyesine neden olmuştur. LDH aktivitesi ile ilgili olarak; toprak üstü kısımdan ve köklerden elde edilen bitki özütlerinin H1299 hücrelerine karşı en yüksek LDH aktivitesi göstermiştir. Elde edilen sonuçlar; bitki köklerinin, kanser hücrelerinde hücre büyümesini konsantrasyon ve zamana bağlı olarak önleyebildiğini göstermiştir.

Anticancer, Antiproliferative and Lactate Dehydrogenase Enzyme Activities of Astragalus elongatus subsp. nucleiferus on Human Cancer Cells

In this study, potential anticancer and antiproliferative activities ofethanol and water extracts from aerial parts and roots of Astragalluselongatus subsp. nucleiferus were evaluated. MTT, trypan blue, andLDH enzyme activity assays were performed to determine cytotoxicityand cell proliferation potentials of the plant extracts against humanA549, H1299, C6 cancer cells, and non-tumorous HUVECs. As resultsof MTT and trypan blue assays, dose-dependent anticancer andantiproliferative effects were observed on tested human lung andbrain carcinoma cells. The water extracts obtained from the rootexerted higher anticancer activity with IC50 values ranging from5.81±0.46 to 18.24±0.12µg/mL than the extracts of aerial parts. Incontrary anticancer and cytotoxicity results, the ethanol extract of theroot was also caused to the highest apoptosis level in a dosedependently. Regarding LDH activity, the plant extracts obtainedfrom aerial parts and roots were demonstrated the highest LDHactivity towards H1299 cells. The obtained results showed that theroots of the plant are able to inhibit cell growth in cancer cells in atime and concentration dependently.

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