Gerze Tavuğu ve Bazı Saf Hat Genotiplerinde Majör Doku Uyumluluğu Gen Kompleksi Polimorfizmi

Bu çalışmada Gerze tavuğu popülasyonuna ait 43 örnek ile ticari safhatlardan oluşturulan 50 örneklik Saf hat popülasyonunun majördoku uyumluluğu gen kompleksi (MHC) bakımından molekülerincelenmesi amaçlanmıştır. Allelik çeşitlilik, MHC içerisinde yer alanMCW0371 ve LEI0258 mikrosatellit lokuslarının PCR yöntemiyardımıyla çoğaltılarak elde edilen DNA fragmentlerinin agaroz jelelektroforezi ile ayrımlanması ile hesaplanmıştır. Gerzepopülasyonunda LEI0258 lokusunda elde edilen allel sayısı, etkiliallel sayısı, gözlenen heterozigotluk ve beklenen heterozigotlukdeğerleri sırasıyla; 16, 10.82, 0.67, 0.91, Saf hat popülasyonunda ise19, 11.84, 0.60, 0.92 olarak bulunmuştur. MCW0371 lokusu içinsırasıyla; Gerze popülasyonunda 3, 2.68, 0, 0.63 ve Saf hatpopülasyonunda 3, 1.96, 0, 0.49 olarak tespit edilmiştir.Popülasyonlar arasında Nei genetik uzaklık ve genetik benzerlikdeğerleri sırasıyla 0.419 ve 0.658 olarak hesaplanmıştır.Popülasyonlar arasında FST değeri 0.068 olarak bulunmuştur. Eldeedilen bulgular, içerdiği allel deseni bakımından Gerze tavuğununözgün bir genetik kaynak olarak korunmasının ve ıslahprogramlarına dahil edilmesinin önemli olduğu sonucuna varılmıştır.

Major Histocompatibility Gene Complex Polymorphism in Gerze Chicken and Some Pure Line Genotypes

The aim of this study was to molecularly investigate the major histocompatibility complex (MHC) polymorphisms of the Gerze chicken population of 43 samples and the commercial pure line population of 50 samples. Allelic diversity was calculated based on PCR amplification of MCW0371 and LEI0258 microsatellite loci in MHC and separation of DNA fragments by agarose gel electrophoresis. In the Gerze population, number of different alleles, number of effective alleles, observed heterozygosity and expected heterozygosity values were calculated as: 16, 10.82, 0.67, 0.91, whereas the values were determined as 19, 11.84, 0.60, 0.92 for the pure line population, respectively for LEI0258 locus. As for MCW0371 locus, the values were calculated as 3, 2.68, 0, 0.63 in the Gerze population and 3, 1.96, 0, 0.49 in the Pure line population, respectively. Nei’s genetic distance and genetic identity values between the populations were calculated as 0.419 and 0.658, respectively. The FST value among the populations was found to be 0.068. The findings revealed that Gerze chicken should be included in breeding programs as a unique genetic resource with its allelic richness.

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