Gülan AYDEMİR, ZİYA DUMLUPINAR, İlker YÜCE, Tuğba BAŞKONUŞ, Selçuk SUNULU, Hüseyin GÜNGÖR

Evaluation of F5 Individuals Obtained from B28×Kunduru-1149 Reciprocal Cross Population by Functional Markers

In the study, B28 and Kunduru-1149 durum wheat genotypes werecrossed as reciprocal in 2012-2013 cropping season. 13 (B28/Kunduru1149 and Kunduru-1149/B28) reciprocal crosses were obtained andwere used as materials at F5 stage. The cross combinations and theparents were screened with 10 DNA markers to determine alleles ofgluten strength (Bx7OE), Yellow rust (Sun104, Xgwm18, Xwgp115 andXgwm47), stem rust (Sun209 and Sun479), high protein ratio(UHW89), powdery mildew (Xgwm66) and leaf rust (Xgwm130). In thestudy, the average polymorphism information content (PIC) wascalculated as 0.98 and the lowest PIC value was obtained fromXwgp115 marker with 0.96, while the rest of the markers had 0.99PIC values. Stem rust resistance allele Sr49 was detected inB28/Kunduru-1149_F5_4 (Sun479) and B28/Kunduru-1149_F5_1(Sun209) genotypes. One of the yellow rust resistance alleles Yr15(Xgwm18) was detected in B28/Kunduru-1149_F5_2 andB28/Kunduru-1149_F5_3 genotypes, while Yr51 (Sun104) wasidentified in B28/Kunduru-1149_F5_3, B28/Kunduru-1149_F5_6,B28/Kunduru-1149_F5_7, Kunduru-1149/B28_F5_2 and Kunduru1149/B28_F5_6 genotypes. A dendrogram was created to determinekinship of the individuals with the parents. The highest geneticsimilarity was observed between B28 / Kunduru-1149_F5_6 andKunduru-1149 / B28_F5_2 genotypes with 0.714, while the mostdiverse ones were Kunduru-1149 and B28/Kunduru_F5_7 with 0.10.

B28×Kunduru-1149 Resiproklu Melez Popülasyonundan Elde Edilen F5 Bireylerinin Fonksiyonel Markörlerle Değerlendirilmesi

Bu çalışmada, B28 ve Kunduru-1149 makarnalık buğday genotipleri 2012-2013 ürün sezonunda resiproklu olarak melezlenmiş ve elde edilen 13 B28/Kunduru-1149-Kunduru-1149/B28 F5 kademesindeki hat materyal olarak kullanılmıştır. Melez kombinasyonları ve ebeveynler gluten dayanıklılığı (Bx7OE), sarı pas (Sun104, Xgwm18, Xwgp115 ve Xgwm47), kara pas (Sun209 ve Sun479), yüksek protein oranı (UHW89), külleme (Xgwm66) ve kahverengi pas (Xgwm130) allelleri bakımından 10 DNA markörü ile taranmıştır. Çalışmada, ortalama polimorfizmi bilgi içeriği (PIC) 0.98 olarak hesaplanmış ve en düşük PIC değeri 0.96 ile Xwgp115 marköründen elde edilirken, diğer markörler 0.99 PIC değerine sahip olmuştur. B28/Kunduru1149_F5_4 (Sun479) ve B28/Kunduru-1149_F5_1 (Sun209) genotiplerinde kara pasa dayanıklılık alleli Sr49 tespit edilmiştir. Sarı pas dayanıklılık allellerinden Yr15 (Xgwm18) B28/Kunduru1149_F5_2 ve B28/Kunduru-1149_F5_3 genotiplerinde bulunurken, Yr51 (Sun104) geni B28/Kunduru-1149_F5_3, B28/Kunduru1149_F5_6, B28/Kunduru-1149_F5_7, Kunduru-1149/B28_F5_2 ve Kunduru-1149/B28_F5_6 genotiplerinde tanımlanmıştır. Ebeveynler ile genotiplerin akrabalıklarını belirlemek için bir dendrogram oluşturulmuştur. En yüksek genetik benzerlik oranı B28 / Kunduru1149_F5_6 ve Kunduru-1149 / B28_F5_2 genotipleri arasında 0.714 olarak tespit edilirken, birbirlerine en uzak genotipler 0.10 benzerlik oranı ile Kunduru-1149 ve B28/Kunduru_F5_7 olmuştur.

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