Vedat TOZYILMAZ, Yusuf CEYLAN, Ali Savaş BÜLBÜL

Anadolu Florasına Ait Bazı Alyssum L. Türlerinin Antimikrobiyal, Antioksidan ve Antibiyofilm Aktivitesinin Belirlenmesi

Doğu Akdeniz’de yayılış gösteren Alyssum L. cinsi, halk tarafından ‘Kuduz otu’ veya ‘Kevke’ olarak bilinir. Alyssum türlerinin çoğu kuraklığa karşı dayanıklı olması ve toprak seçimlerinin olmaması sebebi ile erozyon gibi felaketleri önlemede önemlidir. Ayrıca Alyssum diş otu olarak bilinmekte olup diş ağrılarında, spazm ve mesane taşlarını düşürmede kullanılan bir bitkidir. Bu çalışmada, antibakteriyel ve antifungal aktiviteleri incelemek için ülkemizde doğal olarak yetişen üç bitkiden (Alyssum caricum T.R.Dudley ve Hub.-Mor., Alyssum discolor T.R.Dudley ve Hub.-Mor. ve Alyssum sibiricum Willd.) elde edilen ekstraktlar, disk difüzyon yöntemi ile on yedi bakteri ve iki mantar suşu üzerinde incelenmiştir. Ayrıca, antimikrobiyal aktivite Minimum İnhibisyon Konsantrasyonu (MIC) ve Minimum Bakterisidal Konsantrasyonu (MBC) yöntemi ile desteklenmiştir. Bitki ekstraktlarının antioksidan aktivitelerini araştırmak için DPPH (2,2-difenil-1-pikrilhidrazil) yöntemi, biyofilm oluşumunu önlemek için ise kristal viyole bağlama yöntemi kullanılmıştır. Sonuç olarak, antimikrobiyal çalışmasında en iyi sonucu A. caricum etanol ekstraktı gösterdi. Bitkilerin hiçbiri funguslara karşı etki gösteremedi. Antibiyofilm çalışmasında en iyi sonucu A. sibiricum gösterirken antioksidan çalışmasında ise en iyi aktiviteyi A. caricum ekstraktı gösterdiği tespit edildi.

Determination of Antimicrobial, Antioxidant and Antibiofilm Activity of Some Alyssum L. Species in Anatolian Flora

The Alyssum L. genus which is spread in the Eastern Mediterranean is known by the public as ‘Rabid weed’ or ‘Kevke’. Many of these species are seen as a way to prevent disasters such as erosion, both due to drought resistance and low soil selectivity. Also Alyssum known as ‘Tooth weed’, it has been used to reduce tooth sore, bladder stones and spasm. In this study, in order to examine antibacterial and antifungal activities, extracts obtained from three naturally growing plants (Alyssum caricum T.R.Dudley and Hub.-Mor., Alyssum discolor T.R.Dudley and Hub.-Mor. and Alyssum sibiricum Willd.) in Turkey were examined on seventeen bacteria and two fungal strains by disc diffusion method. In addition, antimicrobial activity was supported by the Minimum Inhibition Concentration (MIC) and Minimum Bactericidal Concentration (MBC) method. DPPH (2,2-diphenyl-1- picrilhydrazyl) method was used to investigate the antioxidant activities of plant extracts, and crystal violet binding method was used to prevent biofilm formation. As a result, A. caricum ethanol extract showed the best effects in antimicrobial activities. None of plants were effective against fungi. A. sibiricum shows the best activity in antibiofilm activity and A. caricum was showed the best result in antioxidant activity.

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