Fatih ŞAHİN, Mustafa ORHAN, Ayça TAŞ TUNA, Onur PALABIYIK, Nevcihan ŞAHUTOĞLU BAL, Sezen Irmak GÖZÜKARA, Özcan BUDAK, Hüseyin ÇAKIROĞLU

Sıçanlarda Hepatik İskemi Reperfüzyon Hasarında Farklı Dozlarda Amantadin'in Akciğer Dokusu Üzerine Etkisi

Amaç: N-Metil D-Aspartat (NMDA) reseptör blokerlerinin çeşitli dokularda iskemi/reperfüzyon (I/R) hasarına karşı koruyucu etkileri olduğu gösterilmiştir. Bu çalışmanın amacı, hepatik I/R hasarında 90 ve 135 mg/kg dozlarındaki amantadinin akciğer üzerindeki etkilerini araştırmaktır. Gereç ve Yöntem: Sıçanlar her rastgele altı gruba ayrıldı: Grup Sham, Grup I/R, Grup Amantadin-90, Grup Amantadin-135, Grup I/R-90 ve Grup I/R-135. I/R'de sol portal triaddaki yapılara 45 dakika süre ile atravmatik vasküler klemp uygulandı ve iskemi sonrası 2 saat reperfüzyon uygulandı. Akciğer dokusunda malondialdehit (MDA), süperoksit dismutaz (SOD) ve katalaz (CAT) enzim düzeyleri ölçüldü ve dokular histopatolojik olarak incelendi. Bulgular: MDA, SOD, CAT düzeyleri açısından gruplar arasında anlamlı fark bulundu (sırasıyla; p

Anahtar Kelimeler:

Amantadin, İskemi Reperfüzyon Hasarı, Akciğer Hasarı, Uzak Organ Hasarı

The Effect of Different Doses of Amantadine on Lung Tissue In Hepatıc Ischemia Reperfusion Injury in Rats

Objective: N-Methyl D-Aspartate (NMDA) receptor blockers have been shown to have protective effects against ischemia/reperfusion (I/R) injury in various tissues. The aim of this study was to investigate the effects of 90 ve 135 mg/kg doses of amantadine on lung in hepatic I/R injury. Method: The rats were randomly divided into six groups: Group Sham, Group I/R, Group Amantadine-90, Group Amantadine-135, Group I/R-90 and Group I/R-135. In I/R, an atraumatic vascular clamp was applied to the structures in the left portal triad for 45 minutes and reperfusion period was 2 hours after ischemia. Malondialdehyde (MDA), superoxide dismutase (SOD) and catalase (CAT) enzyme levels were performed the lung tissue and tissues were examined histopathologically. Result: A significant difference was found between the groups in terms of MDA, SOD, CAT levels (respectively; p

Keywords:

Amantadine, Ischemia reperfusion injury, Lung injury, Remote organ damage,

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ISSN: 1309-3878
Yayın Aralığı: Yılda 3 Sayı
Başlangıç: 2009
Yayıncı: Düzce Üniversitesi Tıp Fakültesi Aile Hekimliği AD adına Yrd.Doç.Dr.Cemil Işık Sönmez