Pankreas Adenokarsinomunda Anjiogenetik Aktivite ve Cox-2 Ekspresyonunun Prognostik Önemi

Amaç: Çeşitli tümörlerde anjiogenez ve siklooksijenaz 2 (COX-2)’nin artmış ekspresyonu arasındaki ilişki çalışılmıştır. Bizim çalışmamızda pankreas duktal adenokarsinomunda COX-2 ekspresyonu ve mikrodamar dansitesinin birbirleri ile ve çeşitli klinikopatolojik parametreler ile ilişkisi araştırılmıştır. Gereç ve Yöntem: Bu çalışmada pankreas adenokarsinomu tanısı almış 61 olgu yer almaktadır. Olgulara ait kesitlere CD31 proteinine karşı geliştirilmiş primer poliklonal antikor ve COX-2 proteinine karşı geliştirilmiş primer poliklonal antikor kullanılarak, streptavidin-biotinimmunperoksidaz yöntemiyle boyama uygulandı. CD31 antikoru ile işaretlenen mikrodamarlar Barth ve arkadaşlarının çalışmalarında tanımladıkları stereolojik yöntem ile sayısal olarak değerlendirildi. COX-2 ekspresyonu, mikrodamar yoğunluğu ve klinikopatolojik parametreler arasındaki ilişki değerlendirildi. Bulgular: Olguların %73.8’inde COX-2 ekspresyonu gösterilmiştir. Çalışmamızda COX-2 reseptör boyanma oranları ve damar parametreleri ve standart prognostik parametrelerin sağ kalım ile ilişkisi saptanmamıştır. Sonuç: Pankreas duktal adenokarsinom tanısı almış olgularımızın çoğunda COX-2 ekspresyonu izlense de COX-2 ile anjiogenez arasında anlamlı bir ilişki saptanmamıştır ve klinikopatolojik faktörler değerlendirildiğinde COX-2’nin aşırı ekspresyonunun prognoza etkisi olmadığı düşünülmüştür

Anahtar Kelimeler:

Pankreas Duktal Adenokarsinom, COX2, CD31

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Objective: The relationship between angiogenesis and hyperexpression of COX-2 in various tumors has been studied. In our study, COX-2 expression in pancreatic ductal adenocarcinoma, and micro vessel density was investigated in relation to each other and with various clinicopathological parameters. Materials and Methods: There are sixty one cases diagnosed with pancreas immunoperoxidase staining technique was applied to sections belonging to the cases, using primary polyclonal antibody developed against the CD31 protein and primary polyclonal antibody developed against the COX-2 protein. Microvessels marked with CD31 antibodies were numerically evaluated by the stereological method described by Barth and his colleagues in their studies. The relationship between COX-2 expression, microvessel density and the clinicopathological parameters were also evaluated. Evidence: COX-2 expression was shown in 73.8% of the cases. No correlation was detected between rates of COX-2 receptor staining, vessel parameters, standard prognostic parameters and survival. Conclusion: Although COX-2 expression was tracked in most cases diagnosed with pancreatic ductal adenocarcinoma, no significant association was found between COX-2 and angiogenesis, and it was thought that hyperexpression of COX-2 doesn't have an impact on prognosis, considering the clinicopathological factors

Keywords:

Pancreas Ductal Adenocarcinoma COX-2, CD31, Microvessel,

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ISSN: 1309-3878
Yayın Aralığı: Yılda 3 Sayı
Başlangıç: 2009
Yayıncı: Düzce Üniversitesi Tıp Fakültesi Aile Hekimliği AD adına Yrd.Doç.Dr.Cemil Işık Sönmez