İsmail DEMİRYILMAZ, İsmayil YILMAZ, Cebrail GÜRSUL, Taha Abdulkadir ÇOBAN, Kemal PEKER, Murat ÇANKAYA, Ilyas SAYAR, Arda ISIK

Ozon Tedavisinin Termal Yanık Yara İyileşmesine Etkisi; Deneysel Bir Çalışma

Amaç: Ozon, hücre içinde, immüno-modülatör unsur ve antioksidan enzim işleticisinde metabolik bir uyarıcı olarak önerilmiştir. Bu çalışma, sıçanlarda yanık yarası iyileşmesinde ozon tedavisinin yararını belirlemek için yapılmıştır. Gereç ve Yöntem: Bu çalışma için 40 adet Wistar tipi sıçan 4 gruba ayrıldı: Bir kontrol grubu (yanık oluşturulmadı, bölgesel ve sistemik unsurlar kullanılmadı), bir yanık grubu (yanık oluşturuldu, ancak sadece salin verildi), bir yanık + gümüş sülfadiazin grubu (21 gün boyunca gümüş sülfadiazin uygulandı), bir yanık + ozon / oksijen karışımı grubu (21 gün boyunca ozon / oksijen karışımı uygulandı) oluşturuldu. Anestezi uygulandıktan sonra, kaynar sıcak suda (15 saniye boyunca 100 ˚C) alüminyum plaka ile hayvanların sırtında ikinci derece yanık (2 cm2 alan) oluşturuldu ve kontrol grubu dışındaki diğer gruplar planlanan sürede bölgesel olarak tedavi edildi. Doku örnekleri yanık hasarından sonraki 7. ve 21. günlerde elde edildi. Doku ve kan örneklerinde biyokimyasal ve histolojik analizler yapıldı. Sonuçlar uygun istatistiksel testlerle değerlendirildi ve ± SD ortalamalar olarak verildi. Bulgular: Histopatolojik hasar düzeyi tüm gruplarda anlamlı olarak farklıydı. Ozon tedavisi ve gümüş sülfadiazin gruplarında fibroz ve inflamasyon seviyeleri, 7 güne göre 21 günde yanık + normal tuzlu su grubuna kıyasla azaldı. Ozon tedavi grubunda, Glikoz 6-fosfat dehidrogenaz aktiviteleri gümüş sülfadiazin ile tedavi edilen gruptan önemli ölçüde yüksekti. Ancak glutatyon redüktaz enzim aktiviteleri, ozon ile tedavi edilen grupta daha düşüktü ve hidroksiprolin konsantrasyonu, 7 ve 21 günde yanık + normal tuzlu su grubuna kıyasla ozon grubunda azaldı. Sonuç: Ozonun, doku hasarını azaltarak ve yanık yara iyileşmesinde antioksidan enzim aktivitesini artırarak iltihaplanma, fibrozis ve granülasyon gelişiminde bozucu bir etkisi bulunmaktadır.

The Effect of Ozone Treatment on Thermal Burn Wound Healing; an Experimental Study

Objective: Ozone has been advised as a metabolic excitative in cell, immunomodulatory agent and antioxidant enzyme actuator. The present study was performedto designate the profit of ozone therapy on the burn wound healing in the rats.Methods: 40 adult Wistar type rats divided into 4 groups: a control group (burn notcreated and topical and systemic agents not used), a burn group (burn created but onlysaline given), a burn+silver sulfadiazine group (silver sulfadiazine was applied for 21days), a burn+ozone/oxygen mixture group (ozone/oxygen mixture was applied for 21days).After anesthetizing, second-degree burn (2 cm2areas) was done on the dorsal ofthe animals byaluminum plate in boiling hot water (100 ˚C for 15 seconds) and othergroups except control group were treated topically, based on the time scheduled.Tissue samples were harvested on day 7st and 21st after burn injury. Biochemical andhistological analyzes were performed in tissue and blood samples. The results wereassessed with appropriate statistical tests and given as Means±SD.Results: The histopathologic damage level was significantly different in all groups.Fibrosis and inflamation levels decreased in ozone treatment and silver sulphadiazinegroups compared to burn+normal saline group on the 21 days according to 7 days. Inthe ozone treatment group, Glucose 6-phosphate dehydrogenase activities weresignificantly higher than the silver sulfadiazine treated group. But glutathionereductase enzyme activities were lover in the ozone treated group and hydroxyprolineconcentration decreased in ozone group compared to burn+normal saline group on 7and 21 days.Conclusions: Ozone has a detractive effect in the development of inflamation,fibrosis, and granulation via decreasing tissue damage and increasing the antioxidantenzyme activity on burn wound healing.

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ISSN: 1309-3878
Yayın Aralığı: Yılda 3 Sayı
Başlangıç: 2009
Yayıncı: Düzce Üniversitesi Tıp Fakültesi Aile Hekimliği AD adına Yrd.Doç.Dr.Cemil Işık Sönmez