GÖRKEM DÜLGER, Tülay TÜTENOCAKLI, BAŞARAN DÜLGER

Methicillin-Dirençli Staphylococcus aureus Bakterilerine Karşı Verbascum thapsus L. Bitkisinin Anti-Staphylococcal Aktivitesi

Bu çalışmada, Verbascum thapsus L. (Scrophulariaceae) bitkisinin yapraklarından elde edilen etanol ekstraktının anti-Staphylococcal aktivitesi Methicillin-Dirençli Staphylococcus aureus (MRSA) bakterilerine karşı Agar-kuyu difüzyon metodu ve mikrodilüsyon metodu ile araştırılmıştır. Etanol ekstraktı Methicillin-Dirençli Staphylococcus aureus (MRSA) bakterilerine karşı (MIC <1024 µg/mL) antibakteriyal bir aktivite göstermiştir. Methicillin ve Gentamicin antibiyotikleri ile karşılaştırıldığında, ekstraktın MRSA strainlerine(suşlarına) karşı oldukça etkili olduğu saptanmıştır. Bu nedenle bitkinin bir anti-Staphylococcal ajan olarak kullanılabilirliği önerilmektedir. (Bu nedenle bitkinin bir anti-Staphylococcal ajan olarak kullanılabilirliği in vitro olarak uygun bulunmuş ve in vivo çalışmaların yapılması gerektiği düşünülmüştür)

Anahtar Kelimeler:

Verbascum thapsus L., Agar-kuyu difüzyon metodu, Mikrodilüsyon metodu, Methicillin-Dirençli Staphylococcus aureus

Anti-Staphylococcal Activity of Verbascum thapsus L. against Methicillin-Resistant Staphylococcus aureus

Present study was conducted to investigate the anti-Staphylococcal activity of ethanol extract obtained from the leaves of Verbascum thapsus L. (Scrophulariaceae) against Methicillin-resistant Staphylococcus aureus (MRSA) by agar–well diffusion method and microdilution method. The ethanol extract showed antibacterial activity against Methicillin-resistant Staphylococcus aureus (MRSA) strains (MIC <1024 µg/mL). When compared with Methicillin and Gentamicin, the extract was more effective against MRSA strains. It is therefore suggested that the extract obtained from V. thapsus could be used as an anti-Staphylococcal agent.

Keywords:

Verbascum thapsus L., Agar-well diffusion method, microdilution method, Methicillin-resistant Staphylococcus aureus,

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ISSN: 1309-3878
Yayın Aralığı: Yılda 3 Sayı
Başlangıç: 2009
Yayıncı: Düzce Üniversitesi Tıp Fakültesi Aile Hekimliği AD adına Yrd.Doç.Dr.Cemil Işık Sönmez