Nimet KILINÇOĞLU, Cevher İlhan CEVHERİ, Cenap CEVHERİ, Hatice Yüsra NAHYA

Kuraklık Stresi Altındaki Pamuk Bitkisine (Gossypium hirsutum L.) Dışsal Prolin Uygulamasının Bazı Fizyolojik Parametreler Üzerindeki Etkisi

Bitkiler kuraklık stresi gibi birçok strese maruz kalmaktadırlar. Araştırmamızda PEG 6000 çözeltisi kullanılmış, 3 adet kuraklık seviyesi oluşturulmuştur (%0, %10, %20 g L-1). Bu oluşturulan kuraklık grupları da kendi içinde 0 (kontrol: prolin çözeltisi uygulanmamış) ve 2 mM prolin çözeltisi uygulanarak iki alt gruba ayrılmıştır. Pamuk bitkisinde farklı kuraklık seviyelerinin oluşturduğu zararlanmaları görüp, dışsal prolin uygulamasının bu zararlanmayı ne kadar iyileştirdiğini görmek amaçlanmıştır. Deneme deseni, saksılarda üç tekrarlamalı olarak yapılmıştır ve deneme deseni iklimlendirme odasında iki kez kurulmuştur. Stres ve kontrol gruplarında klorofil a ve b, karotenoid, toplam çözünmüş protein, toplam çözünmüş karbonhidrat ve bazı besin elementlerinin değerlerinin değişimi incelenmiştir. Artan kuraklık dozuna bağlı olarak klorofil a ve b’nin azaldığı; karotenoidin, toplam çözünmüş protein ve karbonhidratın arttığı; K elementinin arttığı, Ca Mg, Na ve P elementinin azaldığı görülmüştür. Bununla birlikte prolin uygulamasıyla pamuk bitkisindeki kuraklık stresinin olumsuz etkilerinin azaldığı gözlenmiştir.

Anahtar Kelimeler:

Klorofil, protein, karbonhidrat, besin elementleri

The Effect of Exogenous Proline Application on Cotton Plant (Gossypium hirsutum L.) Under Drought Stress on Some Physiological Parameters

Plants are exposed to many stresses such as drought stress. In our study, PEG 6000 solution was used and 3 drought levels were created (0%, 10%, 20% g L-1). These drought groups were also divided into 2 subgroups by applying 0 (control: no proline solution) and 2 mM proline solution. It was aimed to see the damages caused by different drought levels in the cotton plant and to see how much the exogenous proline application remedy this damage. The trial pattern was made in pots with 3 repetitions, the trial was set up in the air conditioning room twice. The changes in the values of chlorophyll a and b, carotenoid, total dissolved protein, total dissolved carbohydrate and some nutritional elements in the stress and control groups were examined. Chlorophyll a and b decreased due to the increasing drought dose; increased carotenoid, total dissolved protein and carbohydrate; It was observed that the K element increased, Ca, Mg, Na and P element decreased. However, it was observed that the negative effects of drought stress in cotton plants were reduced with proline application.

Keywords:

chlorophyll, protein, carbohydrate, nutritional elements,

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