Gülcan AVCI, Aziz BÜLBÜL, Mehmet ÖZDEMİR, A.yasemin GÖKSU EROL

The Investigation of The Effects of Omega 3 Fatty Acids and Sesame Oil on Cyclosporine-A Induced Immunosupressive and Oxidant Effects in Rats

Bu çalışma Wistar Albino rat kullanılarak 15 gün boyunca gerçekleştirildi (n=7). 1)Kontrol: Fizyolojik salin gastrik gavaj ile verildi. 2) CsA: CsA (15 mg/kg/gün) subkutan (sc) olarak uygulandı. 3) CsA+SO grup: CsA (15 mg/kg/day; sc) ve SO (1 ml/kg, gavaj) olarak verildi. 4) CsA+O-3: CsA (15 mg/kg/gün; sc) ve O-3 (100 mg/kg, gavaj) olarak verildi. 5) CsA+SO+O-3: CsA (15 mg/kg/gün; sc), SO (1 ml/kg, gavaj) ve O-3 (100 mg/kg, gavaj) eş zamanlı olarak verildi. 6) SO: sadece SO (1 ml/kg; gavaj) ve 7) O-3: sadece O-3 (100 mg/kg, gavaj). Son uygulamalardan 24 saat sonra ratlar sakrifiye edildi. Biyokimyasal analizler için kan, karaciğer ve böbrek dokuları toplandı. Tüm gruplar arasında NOx bakımında bir fark belirlenmedi. CsA gruba göre diğer tüm gruplarda IL-1α, IL-1β seviyesindeki değişimler önemsizdi. Buna karşın IFN-γ düzeyleri SO ve O-3 hariç diğer tüm gruplarda arttı. Kontrol gruba göre CsA grubunda MDA, GSH, SOD ve CAT düzeylerindeki değişimler hem karaciğer hem de böbrek dokusunda önemsizdi. CsA grubuna göre CsA+O-3 grubunda böbrek dokusu MDA, GSH ve SOD düzeyleri yüksek bulunurken, buna karşın karaciğerdeki değişimler önemsizdi. Bu bulgulara göre, CsA ile birlikte SO ve O-3'ün kullanımı böbreklerde CsA'nın indüklediği oksidatif stresin önlenmesinde yeni bir yaklaşım olabilir.

Anahtar Kelimeler:

Antioksidanlar, Siklosiporin-A, Sitokinler, Omega-3, Susam Yağı

The Investigation of The Effects of Omega 3 Fatty Acids and Sesame Oil on Cyclosporine-A Induced Immunosupressive and Oxidant Effects in Rats

The purpose of this study was to investigate the effects of omega-3 (O-3) and sesame oil (SO) on cyclosporine-A (CsA) induced immunosuppressive and oxidant effects in rats. The study was performed on seven groups for 15 days using Wistar Albino rats (n=7),1) Control: physiological saline was given by gastric gavage, 2) CsA: CsA was administered (15 mg/kg/day) subcutaneously (sc),3) CsA+SO: CsA (15 mg/kg/day; sc) and SO (1 ml/kg, gavage) were given, 4) CsA+O-3: CsA, (15 mg/kg/day; sc) and O-3 (100 mg/kg, gavage) were administered, 5) CsA+SO+O-3: CsA (15 mg/kg/day; sc), SO (1 ml/kg, gavage) and O-3 (100 mg/kg, gavage) were given at the same time, 6) SO: only SO (1 ml/kg; gavage) 7) O-3: only O-3 (100 mg/kg, gavage). 24 h after the last treatments animals were sacrificed. Blood, livers and kidneys were collected for biochemical analysis. There was no difference among all groups in terms of nitric oxide (NOx). Changes in the IL-1α, IL-1β levels were insignificant all other groups compared to CsA group. Nonetheless IFN-γ levels were increased in all groups except SO, O-3 groups. Changes in the level of MDA, GSH, SOD and CAT in both kidney and liver tissues were negligible in the CsA group compared to control group. MDA, GSH and SOD levels in the kidney tissue were determined higher in the CsA groups than CsA+O-3 group, nevertheless changes in the liver were insignificant. Given these findings, use of SO and O-3 with CsA may be a new approach for preventing CsA- induced oxidative stress in kidneys.

Keywords:

Antioxidants, Cyclosporine-A, Cytokines, Omega-3, Sesame Oil,

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