İsmail KÜÇÜKKURT, Damla ARSLAN ACARÖZ, Hasan Hüseyin DEMİREL, Sinan İNCE, Abdullah ERYAVUZ

Ratlarda Gentamisin İle İndüklenmiş Oksidatif Streste Borun Muhtemel Koruyucu Etkisinin Dokularda Araştırılması#

Çalışmada gentamisin maruziyeti ile toksisite oluşturulan ratlarda borun (B) muhtemel koruyucu etkisinin belirlenmesi amaçlandı. Bu amaçla Wistar albino 56 erkek rat kullanıldı. Her grupta 7 hayvan olacak şekilde, kontrol, gentamisin (100 mg/kg i.p.), B-5 (5 mg/kg B, i.p.), B-10 (10 mg/kg B, i.p.), B-20 (20 mg/kg B, i.p.), B-5 + gentamisin (5 mg/kg B ve 100 mg/kg gentamisin, i.p.), B-10 + gentamisin (10 mg/kg B ve 100 mg/kg gentamisin, i.p.), B-20 + gentamisin (20 mg/kg B ve 100 mg/kg gentamisin, i.p.) olarak 8 gruba ayrıldı. Ratlara B uygulaması gentamisin enjeksiyonundan 4 gün önce verilmeye başlandı. Gentamisin uygulamasına 4. gün başlandı ve 12. gün bu uygulama sonlandırıldı. Gentamisin uygulamasının bitişinden itibaren hayvanlara 2 gün daha B verildi. Uygulama sonunda hayvanlardan anestezi altında karaciğer, kalp, beyin, akciğer ve testis dokuları alındı. Gentamisinin karaciğer dokusunda malondialdehid düzeylerini arttırdığı, buna karşın redükte glutatyon düzeylerini, superoksid dismutaz ve katalaz aktivitelerini azalttığı tespit edildi. Histopatolojik incelemede gentamisinin dokularda hasar oluşturduğu gözlendi. Buna karşın, B uygulamasının gentamisin ile indüklenen hasarı azalttığı tespit edildi.

Anahtar Kelimeler:

Bor, Gentamisin, Histopatoloji, Lipid Peroksidasyon, Oksidatif Stres, Rat

Potential Protective Effect of Boron Against Gentamicin-Induced Oxidative Stress on Rat Tissues

The objective of this study was to determine the protective effects of boron on gentamicin induced toxicity in rats. Rats were divided into eight experimental groups containing seven animals in each group. Experimental groups were as follows; control group (fed without B), gentamicin group (100 mg/kg, i.p.), B-5 group (5 mg/kg B, i.p.), B-10 group (10 mg/kg B, i.p.), B-20 group (20 mg/kg B, i.p.), B-5 + gentamicin group (5 mg/kg B and 100 mg/kg gentamicin, i.p.), B-10 + gentamicin group (10 mg/kg B and 100 mg/kg gentamicin, i.p.), and B-20 + gentamicin group (20 mg/kg B and 100 mg/kg gentamicin, i.p.). B was given to rats before four days. Gentamicin was given to rats on the fourth day and finished on the twelfth day. Also, administration of B was completed the fourteenth day. End of the experiment, liver, hearth, brain, lung, and testis tissues were collected from animals under anaesthesia. Administration of gentamicin increased malondialdehyde levels whereas decreased reduce glutathione levels, superoxide dismutase and catalase activities in the liver. Histopathologically, damages were detected in the tissues of gentamicin group. However, treatment of boron resulted in a reversal of gentamicin-induced damages.

Keywords:

Boron, Gentamicin, Histopathology, Lipid Peroxidation, Oxidative Stress, Rat,

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