Ratlarda Fruktoz ile Oluşturulmuş Metabolik Sendromun Böbrek Histolojisi Üzerine Etkileri

Metabolik sendrom pandemiye doğru ilerleyen, insülin direnci, abdominal obezite, dislipidemi, diabetes mellitus, hipertansiyon ve koroner arter hastalığı (KAH) ile karakterize ölümcül bir endokrinopatidir. Sunulan çalışamada metabolik sendromun rat böbreklerinde oluşturduğu histomorfolojik ve histokimyasal değişimlerin araştırılması amaçlanmıştır. Bu amaçla materyal kontrol grubunda 10, metabolik sendrom grubunda 15 rat içerecek şekilde iki gruba ayrıldı. Kontrol grubundaki ratlara 16 hafta süresince çeşme suyu verilirken, metabolik sendrom grubundaki ratlara içme suyu olarak % 20 fruktoz çözeltisi verildi. Deneme sürecinin sonunda böbreklerden alınan kesitlerde histolojik görünümü ve histomorfolojik değişimleri belirlemek amacıyla Crossman’s üçlü boyama metodu, histokimyasal analiz amacıyla Periyodik Asit Schiff Reagent (PAS) boyama metodu uygulandı. Elde edilen veriler tubulus proksimalis çapı ve glikojen yoğunluğu ile glomerular mezangial matriks yoğunluğunun arttığını, korpuskulum renis çapı, Bowman aralığı genişliği ve çıkan henle çapının azaldığını göstermiştir. Sonuç olarak, metabolik sendromun böbrek histolojisini olumsuz yönde etkileyerek renal hasara sebep olabileceği ortaya konmuştur.

Anahtar Kelimeler:

Metabolik sendrom, fruktoz, histoloji, böbrek, rat

Effects of Fructose-Induced Metabolic Syndrome on Kidney Histology in Rats

Metabolic syndrome is a fatal endocrinopathy, which is progressed towards pandemic, and characterized by insulin resistance, abdominal obesity, dyslipidemia, diabetes mellitus, hypertension and coronary artery disease (CAD). In the present study, it is aimed to investigate the histomorphological and histochemical changes caused by the metabolic syndrome in rat kidneys. For this purpose, the material was divided into two groups: 10 rats in the control group and 15 rats in the experimental group. During 16 weeks, while tap water was given to control group rats, water including 20% fructose solution was given to experimental group rats as drinking water. At the end of the study, Crossman's triple staining method was performed to determine the histological appearance and histomorphological changes in the sections taken from the kidneys. Periodic Acid Schiff Reagent (PAS) staining method was performed for histochemical analysis. The results of the study showed that the tubulus proximalis diameter, tubulus proximalis glycogen density, and glomerular mesangial matrix density increased, corpusculum renis diameter, width of cavum glomeruli and ascending limb of Henle’s loop diameter decreased. In conclusion, it is demonstrated that metabolic syndrome may adversely affect kidney histology and cause renal damage.

Keywords:

Metabolic syndrome, fructose, histology, kidney, rat,

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