Bu çalışmanın amacı, kliniğimize getirilen ve kistik endometriyal hiperplazi (KEH)-pyometra kompleks tanısı konulan köpeklerde serum adenozin deaminaz aktivitesinin belirlenmesi ve sonuçların kan lenfosit ve monosit sayılarıyla ilişkisinin araştırılmasıdır. Çalışmada açık serviks KEHpyometrası bulunan 18 köpek ve diöstrus döneminin ortasında olan 7 sağlıklı köpek olmak üzere toplam 25 köpek kullanıldı. KEH-pyometra tanısı klinik, ultrasonografik ve hematolojik bulgulara dayanılarak konuldu. Kontrol köpeklerinin diöstrusun ortasında olduğu sitolojik muayeneyle belirlendi. Ovaryohisterektomi yapılmadan önce kan örnekleri alındı ve operasyon sonrası histopatolojik incelemeler için doku örnekleri alındı. ADA aktivitesi spektrofotometrik olarak ölçüldü. Serum ADA aktivitesi KEH-pyometralı köpeklerde 5.99±0.31 U/l, diöstrustaki kontrol köpeklerinde ise 3.50±0.64 U/l olarak belirlendi. Ortalama serum ADA aktivitesi (KEH)-pyometra kompleks tanısı konulan köpeklerde sağlıklı köpeklere göre daha yüksek bulundu (p≤0.01). Sonuç olarak, KEH-pyometra kompleksinin köpeklerde hücre aracılı bağışıklık sistemini uyararak serum ADA aktivitesinde yükselmeye neden olduğu ve serum ADA aktivitesinin değerlendirilmesinin bu hastalığın tanısında ve tedavisinin izlenmesinde yararlı bir parametre olabileceği kanısına varılmıştır. ●●● Determination of Serum Adenosine Deaminase Activity in Bitch Diagnosed as Cystic Endometrial Hyperplasia (CEH)-pyometra Complex S U M M A R Y The purpose of the study was to investigate serum ADA activity and blood lymphocyte and monocyte numbers in the bitch diagnosed as cystic endometrial hyperplasia (CEH)-pyometra complex. Twenty five female dogs were involved in the study. Samples from 18 bitches with open cervix CEHpyometra and 7 mid-diestrus bitches were used. The diagnosis of CEHpyometra was based on clinical, ultrasonographical and haematological findings. Mid-diestrus control bitches were diagnosed by vaginal cytologic examination. Blood samples were taken before the operation and tissue samples were collected at the time of surgery from the uteri of 18 adult bitch diagnosed as cystic endometrial hyperplasia (CEH)-pyometra complex and of 7 healthy middiestrus dogs presented for ovariohysterectomy for histopathological investigation. Serum ADA activity was measured by spectrophotometrically and determined to be 5.99±0.31 U/l in bitches with CEH-pyometra and 3.50±0.64 U/l in mid-diestrus bitches. Mean serum ADA activity were higher in bitches with CEH-pyometra complex compared with the concentrations in healthy mid-diestrus dogs (p≤0.01). It was concluded that CEH-pyometra complex results in increase in serum ADA activity by cell-mediated immune system and evaluation of serum ADA activity could be an useful parameter for diagnosis and treatment follow-up of this complex.

The purpose of the study was to investigate serum ADA activity and blood lymphocyte and monocyte numbers in the bitch diagnosed as cystic endometrial hyperplasia (CEH)-pyometra complex. Twenty five female dogs were involved in the study. Samples from 18 bitches with open cervix CEHpyometra and 7 mid-diestrus bitches were used. The diagnosis of CEHpyometra was based on clinical, ultrasonographical and haematological findings. Mid-diestrus control bitches were diagnosed by vaginal cytologic examination. Blood samples were taken before the operation and tissue samples were collected at the time of surgery from the uteri of 18 adult bitch diagnosed as cystic endometrial hyperplasia (CEH)-pyometra complex and of 7 healthy middiestrus dogs presented for ovariohysterectomy for histopathological investigation. Serum ADA activity was measured by spectrophotometrically and determined to be 5.99±0.31 U/l in bitches with CEH-pyometra and 3.50±0.64 U/l in mid-diestrus bitches. Mean serum ADA activity were higher in bitches with CEH-pyometra complex compared with the concentrations in healthy mid-diestrus dogs (p≤0.01). It was concluded that CEH-pyometra complex results in increase in serum ADA activity by cell-mediated immune system and evaluation of serum ADA activity could be an useful parameter for diagnosis and treatment follow-up of this complex