Ankara Bölgesinden Toplanan Bal Arısı Kovanı Ürünlerinin Antioksidan Özelliklerinin ve Toplam Fenolik ve Flavonoid İçeriklerinin Değerlendirilmesi

Bu çalışmada, arı ürünleri olan multifloral bal, arı ekmeği, arı poleni ve erkek arı larvası (apilarnil)'in toplam fenolik (TP) ve toplam flavonoid (TF) profilleri ortaya konuldu. Bunun yanı sıra söz konusu örneklerin antioksidan aktiviteleri, 2,2′-azinobis (3-ethylbenzothiazolin)-6-sulfphonate (ABTS) testi ve ferric reducing antioxidant power (FRAP) testi ve 1,1-diphenyl-2-picrylhydrazy (DPPH) testi metotları kullanılarak belirlendi. Çalışmada materyalini Ankara yöresindeki 10 bal arısı kovanından toplanan bal, arı ekmeği, arı poleni ve apilarnil örnekleri oluşturdu. Yapılan analizlerin sonucunda bal arısı ürünlerinin toplam fenolik içerik düzeyine göre sıralaması arı poleni > arı ekmeği > bal > apilarnil olarak bulunurken, bal arısı ürünlerinin toplam flavonoid içeriği düzeyine göre sıralaması ise şu şekildedir: arı poleni > arı ekmeği > apilarnil > bal. Bal arısı kovan ürünlerinin DPPH, FRAP ve ABTS analizine göre sıralaması bal > arı poleni > arı ekmeği > apilarnil olarak belirlendi. Sonuç olarak balın en yüksek antioksidan aktivite düzeyi balın içerdiği diğer biyoaktif kompleks maddelerin sinerjistik antioksidan etkileri olarak değerlendirilmiştir ve bu nedenle balın antioksidan aktivite düzeyini artıran biyoaktif kompleks maddelerin ileriki çalışmalarda değerlendirilmesi gerektiği kanaatindeyiz.

Anahtar Kelimeler:

ABTS assay, Ankara yöresi, DPPH assay, FRAP assay, Toplam fenolik içerik, Toplam flavonoid içerik

Evaluation of Antioxidant Properties and Total Phenolic and Flavonoid Contents of Honey Bee Hive Products Collected from the Ankara Region

In this study, the total phenolic (TP) and total flavonoid (TF) profiles of multifloral honey, bee bread, bee pollen, and drone larvae (apilarnil), which are among the products of bee hives, were determined. In addition, the antioxidant activities of the aforementioned products were determined by 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assay, ferric reducing antioxidant power (FRAP) assay, and 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay methods. Honey, bee bread, bee pollen, and apilarnil samples collected from 10 honey bee hives in the Ankara region were the material of the study. As a result of the analysis, TP content levels were found to be in the order of bee pollen > bee bread > honey > apilarnil, while TF contents were in the order of bee pollen > bee bread > apilarnil > honey. Considering the results of the DPPH, FRAP, and ABTS assays, the levels of activity were determined in the order of honey > bee pollen > bee bread > apilarnil. The highest antioxidant activity level determined in honey was concluded to be the result of synergistic antioxidant effects of other bioactive complex substances contained in honey. Therefore, we believe that bioactive complex substances that increase the antioxidant activity level of honey should be evaluated in future studies.

Keywords:

ABTS assay, FRAP assay, DPPH assay, Total phenol contents, Total flavonoid contents, Ankara region,

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