Semiha ORHAN, Tuna DEMİRDAL, Fatih Mehmet BİRDANE, Mustafa KABU, Halit Buğra KOCA, Neşe DEMİRTÜRK

STAPHLOCOCCUS AUREUS İLE OLUŞTURULAN DENEYSEL İNTRAABDOMİNAL KAYNAKLI BAKTERİYEMİ MODELİNDE BETA GLUKANIN İMMÜNOMODULATÖR ETKİSİNİN ARAŞTIRILMASI

AMAÇ: Bu çalışmanın amacı S. aureus ile oluşturulan deneysel intraabdominal kaynaklı bakteriyemi modelinde beta glukanın immünomodulatör etkisini araştırmaktır.GEREÇ VE YÖNTEM: Wistar albina sıçanlardan 30’u rastgele 4 gruba ayrıldı. Sırasıyla Staphylococcus aureus bakteriyemisi (SAB), sefazolin ile tedavi, beta glukan tedavi, beta glukan ve sefazolin tedavi grupları oluşturuldu. İntraabdominal yolla verilen 12x10 8 cfu/ml 1 cc S. aureus verildikten sonra intraabdominal 4 mg/kg beta glukan ve 100 mg/kg sefazolin verildi. 2 saat sonra 0,5 cc kan alınarak kan kültür şişesine ekim yapıldı. 4-6-8. saat sonra Tümör nekroz faktörü alfa (TNF-α), İnterlökin-1( IL-1), İnterlökin-6 (IL-6), İnterferon gama (IFN-γ) düzeyleri araştırıldı.BULGULAR: Biyokimyasal analizlere göre; çalışma sonunda beta glukan 6. saatte IFN-γ’yı arttırdığı, ancak 4. ve 8. saatlerde arttırmadığı görüldü. Sefazolin ile birlikte verildiğinde 6. saatte bu artış daha belirgin olmaktadır. Ancak sefazolin verilen grupta IFN-γ değerleri beta glukandan daha yüksek düzeyde saptandı. Serum TNF-α düzeyleri değerlendirildiğinde, beta glukan verilen grupta 8. saatte TNF-α düzeyinde bir baskılanma saptansa da SAB grubundan daha yüksek olarak bulundu. Serum IL-1 düzeyleri değerlendirildiğinde, beta glukan verilen grupta 4-6 ve 8. saatlerde IL-1 düzeyleri SAB grubuna göre daha yüksekti. Beta glukana sefazolin eklenen grupta 8. saatte IL-1 düzeylerinde azalma tesbit edildiyse de, bu düzeyler SAB grubundan yüksek saptandı. Serum IL-6 seviyesi değerlendirildiğinde, beta glukan verilen grupta SAB grubuna göre ilk 8 saatte IL-6 salınımında artış saptandı. Beta glukan sefazolinle birlikte uygulandığında ise IL-6 artışının ilk 8. saatte en yüksek düzeye ulaştığı görüldü.SONUÇ: Bu deneysel intraabdominal kaynaklı bakteriyemi modeli beta glukanın özellikle ilk saatlerde TNF-α, IL-1 üretimini baskılamadığını, IL-6, IFN-γ salınımını arttırdığını göstermiştir. Bu sonuçlara göre deneysel intraabdominal kaynaklı bakteriyemi tedavisinde beta glukan kullanımının yararı konusunda anlamlı bilgilere ulaşılamadı.

Anahtar Kelimeler:

beta glucan, cytokines, sepsis, staphylococcus aureus

INVESTIGATION OF THE IMMUNOMODULATOR EFFECT OF BETA GLUCAN IN THE EXPERIMENTAL INTRAABDOMINAL BACTERIEMIA MODEL WITH STAPHYLOCOCCUS AUREUS

OBJECTIVE: The purpose of this study is to investigate the immunomodulatory effect of beta glucan in an experimental model of intraabdominal bacteremia induced by S. aureus.MATERIAL AND METHODS: Thirty Wistar albino rats were randomly divided into four groups. Staphylococcus aureus bacteremia (SAB), treatment with cefazolin, treatment with beta glucan, treatment with both cefazolin and beta glucan groups were constituted respectively. 4 mg/kg beta glucan and 100 mg/kg cefazolin were given after 12x10 8 cfu/ml 1 cc S. aureus was given intraabdominally. After two hours, 0.5 cc blood was drawn and put into blood culture bottles. Levels of Tumor necrosis factor-alpha (TNF-α), interleukin-1 (IL-1), interleukin-6 (IL-6), and Interferon gamma (IFN-γ) were evaluated after 4th, 6th and 8th hours.RESULTS: According to the biochemical analyses; At the end of the study it was seen that beta glucan increased the level of IFN-γ at 6th hour, but did not at 4th and 8th hours. This increase became more apparent at 6 th hour when it was given with cefazolin. However IFN-γ levels were found to be higher in the group which cefazolin was given than beta glucan was. As the level of serum TNF-α was evaluated, although there was a supression at 8th hour, it was found to be higher in the group which beta glucan was given than the SAB group. Serum IL-1 levels were higher at 4th, 6th and 8th hours in the group beta glucan was given than the SAB group. Although a decline in IL-1 level was detected in the group which cefazolin was added to beta glucan at 8th hour, this level was found to be higher than the SAB group. When the serum IL-6 level was evaluated, an increase in release of IL-6 was found in the group beta glucan was given in the first 8 hours when it was compared with SAB group. When beta glucan was given with cefazolin, it was observed that IL-6 increased to the highest level at 8th hour.CONCLUSIONS: This experimental intra-abdominal bacteremia model, demonstrated that beta glucan did not supress the production of TNF-α and IL-1, and increased the release of IL- 6 and IFN-γ, especially in the first hours. According to these results, no significant knowledge could be obtained about the benefit of using beta glucan in the treatment of experimental model of intraabdominal bacteremia.

Keywords:

Bacteremia, Beta-Glucans, Cytokines, Staphylococcus aureus,

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