ERGİN MURAT ALTUNER, ILGAZ AKATA, KEREM CANLI

In vitro antimicrobial activity screening of bovista nigrescens Pers.

Bu çalışmanın amacı, Bovista nigrescense ait etanol ekstraktlarının 17 bakteri, 1 mantar suşuna karşı gösterdiği antimikrobiyal aktivitenin incelenmesidir. Yomra, Çamlıyurt, Trabzon, Türkiyeden toplanan B. nigrescens örnekleri kurutulduktan sonra etanol ile ekstraksiyona tabi tutulmuştur. Bu ekstraktların; Bacillus subtilis ATCC 6633, Candida albicans ATCC 10231, Enterobacter aerogenes ATCC13048, Enterococcus durans, Enterococcus faecalis ATCC 29212, Enterococcus faecium, Escherichia coli ATCC 25922, Escherichia coli CFAI, Klebsiella pneumoniae, Listeria monocytogenes ATCC 7644, Salmonella enteritidis ATCC 13075, Salmonella infantis, Salmonella kentucky, Salmonella typhimurium SL 1344, Staphylococcus aureus ATCC 25923, Staphylococcus carnosus MC1.B, Staphylococcus epidermidis DSMZ 20044 ve Streptococcus agalactiae DSMZ 6784 gibi geniş yelpazedeki suşlara karşı in vitro antimikrobiyal aktiviteleri disk difüzyon yöntemi kullanılarak incelenmiştir. B. nigrescensin etanol ekstraktlarının bazı gram negatif ve gram pozitif mikroorganizmaya etki ettiği gözlenmiştir. Bu çalışmanın sonucu olarak B. nigrescensin etanol ekstraktarının bazı mikroorganizmalar üzerine aktivite gösterdiği, ancak B. subtilis, K. pneumoniae ve S. carnosus üzerine gösterdiği etkinin dikkat çekici olduğu sonucuna varılmıştır.

Bovista nigrescens Pers.in in vitro antimikrobiyal etki taraması

The aim of this study is to determine the antimicrobial activity of Bovista nigrescens ethanolic extracts against 17 bacterial and 1 fungal strains. B. nigrescens samples which were collected from Yomra, Çamlıyurt, Trabzon, TURKEY were air dried and extracted with ethanol. Extracts were investigated for in vitro antimicrobial activity against a wide range of strains such as Bacillus subtilis ATCC 6633, Candida albicans ATCC 10231, Enterobacter aerogenes ATCC13048, Enterococcus durans, Enterococcus faecalis ATCC 29212, Enterococcus faecium, Escherichia coli ATCC 25922, Escherichia coli CFAI, Klebsiella pneumoniae, Listeria monocytogenes ATCC 7644, Salmonella enteritidis ATCC 13075, Salmonella infantis, Salmonella kentucky, Salmonella typhimurium SL 1344, Staphylococcus aureus ATCC 25923, Staphylococcus carnosus MC1.B, Staphylococcus epidermidis DSMZ 20044 and Streptococcus agalactiae DSMZ 6784 by using the disc diffusion method. It is observed that ethanolic extract of B. nigrescens has antimicrobial activity against several gram positive and gram negative microorganims tested. As a result of the study, it could be concluded that ethanolic extract of B. nigrescens is active against several microorganisms but its antimicrobial activity is notable especially against B. subtilis, K. pneumoniae and S. carnosus.

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Agrahar-Murugkar D., Subbulakshmi G. 2005. Nutritional value of edible wild mushrooms collected from Khasi hills of Meghalaya. Food Chemistry, 89, 599-603.
Altuner E.M., Akata I. 2010 Antimicrobial Activity of Some Macrofungi Extracts. Sakarya Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 14, 45-49.
Altuner E.M., Çeter T., İşlek C. 2010. Investigation of antifungal activity of Ononis spinosa L. ash used for the therapy of skin infections as folk remedies. Mikrobiyoloji Bülteni, 44, 633-639.
Altuner E.M., Çetin B. 2009. Antimicrobial activity of Thuidium delicatulum (Bryopsida) extracts. Kafkas Üniversitesi, Fen Bilimleri Enstitüsü Dergisi, 2, 85-92.
Altuner E.M., Çetin B. 2010. Antimicrobial activity of Tortella tortulosa (Hedw.) Limpr. Extracts. Kastamonu University, Journal of Forestry, 10, 111-116.
Andrews J.M. 2003. BSAC standardized disc susceptibility testing method (version 6). Journal of Antimicrobial Chemotherapy, 60, 20-41.
Baytop T. 1999. Geçmişte ve Bugün Türkiye’de Bitkiler ile Tedavi. Nobel Tıp Kitabevi Ltd Şti. İstanbul.
Bobek P., Ozdyn L., Kuniak, L. 1995. The effect of oyster (Pleurotus ostreatus) its ethanolic extracts and extraction residues on cholesterol levels in serum lipoproteins and liver of rat. Nahrung, 39, 98-99.
Bobek P., Galbavy S. 1999. Hypocholesterolemic and anti-atherogenic effect of oyster mushroom (Pleurotus ostreatus) in rabbit, Nahrung, 43, 339-342.
Bonatti M., Karnopp P., Soares H.M., Furlan S.A. 2004. Evaluation of Pleurotus ostreatus and Pleurotus sajor-caju nutritional characteristics when cultivated in different lignocelluloses wastes. Food Chemistry, 88, 425-428.
Breitenbach J., Kränzlin, 1986. F. Fungi of Switzerland. Vol: 2, Nongilled Fungi. Verlag Mykologia CH-6000 Luzern 9, Switzerland. Burk W.R. 1983. Puffball usages among North American Indians. Journal of Ethnobiology, 3, 55-62.
Calonge F.D. 1998. Gasteromycetes, I. Lycoperdales, Nidulariales, Phallales, Sclerodermatales, Tulostomatales. Volume 3. Real Jardin Botanico, Stuttgart.
Cheung L.M., Cheung P.C.K. 2005. Mushroom extracts with antioxidant activity against lipid peroxidation. Food Chemistry, 89, 403-409.
Clardy J., Walsh C. 2004. Lessons from natural molecules. Nature, 432, 829-837.
Cos P. Vlietinck, A.J., Vanden Berghe D., Maes L. 2006. Anti-infective potential of natural products: How to develop a stronger in vitro ‘proof-of-concept’. Journal of Ethnopharmacology, 106, 290-302.
Dülger B., Şen, F., Gücin F. 1999. Russula delica Fr. makrofungusunun antimikrobiyal aktivitesi. Turkish Journal of Biology, 23, 127- 133.
Dulger B., Suerdem T.B., Yesilyurt D., Hacioglu N., Camdeviren A. 2005. Evaluation of Antimicrobial Activity of the Macrofungus Phellinus torulosus. Journal of Biological Sciences, 5, 436-439.
Dulger B., Yayintas O.T., Gonuz A. 2005. Antimicrobial activity of some mosses from Turkey. Fitoterapia, 76, 730-732.
Gunde-Cimerman N. 1999. Medicinal value of genus Pleurotus (Fr) P. Karst. (Agaricles S.R. Basidiomycetes). International Journal of Medicinal Mushrooms, 1, 69-70.
Hammer K.A., Carson C.F., Riley, T.V. 1999. Antimicrobial activity of essential oils and other plant extracts. Journal of Applied Microbiology, 86, 985 - 990.
Imtiaj A., Lee T.S. 2007. Screening of antibacterial and antifungal activities from Korean wild mushrooms. World Journal of Agricultural Sciences, 3, 316-321.
Jones F.A. 1996. Herbs - useful plants. Their role in history and today. European Journal of Gastroenterology and Hepatology, 8, 1227-1231.
Keleş O., Ak S., Bakırel T., Alpınar K. 2001. Türkiye’de yetişen bazı bitkilerin antibakteriyel etkisinin incelenmesi, Turkish Journal of Veterinary and Animal Sciences, 25:559-565.
Mahasneh A.M. and El-Oqlah A.A. 1999. Antimicrobial activity of extracts of herbal plants used in the traditional medicine of Jordan. Journal of Ethnopharmacology, 64, 271-276.
McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E., Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R., Wilson, R.K. 2001. Complete genome sequence of Salmonella enterica serovar Typhimurium LT2. Nature, 413, 852-856.
Nikaido H. 1998. Antibiotic resistance caused by gram-negative multidrug efflux pumps. Clinical Infectious Diseases, 27, 32-41.
Okeke I.N., Laxmaninarayan R., Bhutta Z.A., Duse A.G., Jenkins P., O’Brien T.F., PablosMendez A., Klugman, K.P. 2005. Antimicrobial resistance in developing countries. Part 1: recent trends and current status. Lancet Infectious Diseases, 5, 481-493.
Ooi V.E.C. 2000. Medicinally important fungi. In: Van Griensven (Ed.) Science and Cultivation of Edible Fungi. Balkerna, Rotterdam.
Paudel B., Bhattarai H.D., Lee J.S., Hong S.G., Shin H.W., Yim, J.H. 2008. Antibacterial Potential of Antarctic Lichens against Human Pathogenic Gram-positive Bacteria. Phytotheraphy Research, 22, 1269-1271.
Quereshi S., Pandey A.K., Sandhu S.S. 2010. Evaluation of antibacterial activity of different Ganoderma lucidum extracts. People’s Journal of Scientific Research, 3, 9-13.
Silici S., Koc, A.N. 2006. Comparative study of in vitro methods to analyse the antifungal activity of propolis against yeasts isolated from patients with superficial mycoses. Letters in Applied Microbiology, 43, 318-324.
Syed G.W., Syed A.S., Oh L.A. 2010. Risk Evaluation Under Various Speculations of Antibiotic Usage; A Cohort Survey Among Outpatients of Pinang, Malaysia. Eur J Gen Med, 7, 303-309.
URL1. 2007. Fraser S.L., Arnett M., Sinave C.P. Enterobacter infections, Available from: http://www.emedicine.com/med/topic678.htm [Accessed 01 January 2012].
URL2. 2007. WHO (World Health Organization), The world health report 2007: A safer future: global public health security in the 21st century, 2007, http://www.who.int/whr/2007 [Accessed 01 January 2012].
Vaara M. 1993. Antibiotic-supersusceptible mutants of Escherichia coli and Salmonella typhimurium, Antimicrob Agents Chemother, 37, 2255-2260.
Villegas M.V., Quinn, J.P. 2004. An update on antibiotic-resistant gram-negative bacteria. Infections in Medicine, 21, 595-599.
Wasser S.P., Weis A.L. 1999a. Medicinal properties of substances occurring in higher basidiomycete mushrooms: Current perspective. International Journal of Medicinal Mushrooms, 1, 31-62.
Wasser S.P., Weis, A.L. 1999b. Therapeutic properties of substances occurring in higher basidiomycete mushrooms: A modern perspective. Critical Reviews of in Immunology, 19, 65-96.