Himantoglossum robertianum (Loisel.) P.Delforge’un Asimbiyotik Tohum Çimlendirilmesinde Sıcaklığın Etkisi

Çalışmanın amacı: Uluslararası sözleşmeler tarafından korunmasına rağmen, her yıl doğadan milyonlarca orkide yumrusu sökülmekte ve doğal yayılış alanları yok edilmektedir. Bu türlerden biri olan Himantoglossum robertianum, aşırı toplama nedeniyle yok olma tehdidi altındadır ve korunması için önlemler alınmalıdır. Ex-situ korumanın sağlanması için türlerin asimbiyotik ortamda çoğaltılması zorunludur. H. robertianum'da optimum çimlenme sıcaklığı ile ilgili bir çalışma bulunmamaktadır. Bu çalışmanın amacı, türün asimbiyotik koşullarda doku kültürü ortamında ve farklı sıcaklıklarda çimlenme koşullarını belirlemektir. Çalışma alanı: Çalışma Bursa Teknik Üniversitesi Orman Fakültesi Orman Mühendisliği Bölümü Silvikültür Laboratuvarlarında gerçekleştirilmiştir. Materyal ve yöntem: Çalışmada H. robertianum tohumları kullanılmıştır. Tohumlar dört farklı sıcaklıkta beş tekerrürlü olarak çimlendirilmiştir. Çimlendirme ortamı olarak Sığma-Phytamax P-6668 kullanılmış ve karanlık koşullarda 275 gün süreyle yürütülmüştür. Temel sonuçlar: En yüksek çimlenme 20°C’de %23.8 olarak gerçekleşirken, 10°C'de çimlenme elde edilememiştir. İlk 18 haftada 25°C'de çimlenme hızı yüksek iken daha sonra 20°C'de çimlenme hızlanmıştır. Araştırma vurguları: Bu sonuçlar, H. robertainum tohumlarının çimlenmesinde sıcaklığın önemli bir faktör olduğunu göstermektedir

Anahtar Kelimeler:

Himantoglossum robertianum, Asimbiyotik Çimlenme, Sıcaklık

Effects of Temperature on Asymbiotic Seed Germination of Himantoglossum robertianum (Loisel.) P.Delforge

Aim of study: Despite protection by international agreements, millions of orchid tubers are harvested from their natural distribution areas each year. Of these species, Himantoglossum robertianum is locally threatened due to overharvesting and requires precautionary measures to ensure its protection. Reproduction of the species in an asymbiotic environment is imperative for providing ex-situ protection. There are no studies on optimum germination temperature in H. robertianum. This study aimed to germinate H. robertianum seeds in-vitro under asymbiotic conditions. Area of study: The study was carried out at the Silviculture Laboratories of Bursa Technical University, Faculty of Forestry, Department of Forestry Engineering. Material and methods: H. robertianum seeds were used in the study. Seeds were germinated in five replications at four different temperatures (10, 15, 20, and 25°C (± 0.5°C). The study was conducted for 275 days under dark conditions with Sıgma-Phytamax P-6668 used as the medium. Main results: The highest germination was 23.8% at 20°C and germination was not obtained at 10°C. While germination was faster at 25°C in the first 18 weeks, germination accelerated at 20°C after 18 weeks. Highlights: These results indicate that temperature is an important factor in the germination of H. robertainum seeds.

Keywords:

Himantoglossum robertianum, Asymbiotic Germination, Temperature,

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