Rabia SARIKAYA, Şükran ÇAKIR, Kemal SOLAK

GIDALARDAKİ KORUYUCU MADDELERİN DROSOPHİLA MELANOGASTER’DE MWHXFLR ÖMÜR UZUNLUĞUNA ETKİSİ

Bu çalışmada, dört gıda katkı maddesinin sodyum nitrit, sodyum nitrat, potasyum nitrit ve potasyum nitrat Drosophila melanogaster’de mwhxflr ömür uzunluğuna etkisi araştırılmıştır. Deneylerde Drosophila melanogaster’in multiple wing hair ve flare ırkları kullanılmıştır. Üçüncü dönem Trans heterozigot larvalara test kimyasalları 75 mM’lık konsantrasyonlarda uygulanmıştır. Drosophila melanogaster’in mwh x flr hayat tablosu verileri değerlendirilmiş ve en düşük ortalama ömür uzunluğu sodyum nitrit uygulanan grupta bulunmuştur. Kimyasallar ömür uzunluğuna etkilerine göre şu şekilde sıralanabilirler: Potasyum nitrit, sodyum nitrat, potasyum nitrat ve kontrol grubu. Ayrıca tüm gruplardaki deney sonuçları dişi bireylerin erkek bireylerden daha uzun yaşadıklarını göstermektedir. Nitrit içeren uygulama gruplarındaki ortalama ömür uzunluğunun nitrat içeren gruplara oranla daha kısa olduğu belirlenmiştir.

Anahtar Kelimeler:

Drosophila melanogaster mwh X flr , Ömür uzunluğu, Gıda koruyucuları, Yaşlanma

THE EFFECTS OF FOOD PRESERVATIVES ON THE LONGEVITY OF DROSOPHILA MELANOGASTER MWHXFLR

In this study, the effects of four food preservatives sodium nitrate, sodium nitrite, and potassium nitrate and potassium nitrite on the longevity of Drosophila melanogaster were investigated. In the experiments mwh and flr strains of Drosophila melanogaster were used. Third-instars larvae trans-heterozygous were treated of the test compounds at a concentration of 75 mM. Life span data of Drosophila melanogaster mwh x flr were evaluated and the lowest mean life span was found to be groups of sodium nitrite. Chemicals used were ranked as potassium nitrite, sodium nitrate, potassium nitrate and control group according to effects of life span. In addition, experimental result show that all of the groups female survived longer than male. The treatment groups which contain nitrite of mean life span was determined shorter than the treatment groups which contain nitrate.

Keywords:

Drosophila melanogaster mwh X flr , Longevity, Food preservatives, Aging.,

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Bozcuk, A.N., (1981). Genetics of Longevity in Drosophila V. The specific and hybridised effects of rolled, speia, ebony and eyeless autosomal mutants, Experimental Gerontology, 16:415-427. Comfort, A., (1966). The prevention of aging in cells, The Lancet, 17: 1325-1329.
Pearl, R.. and Miner, J.R., (1935). Experimental studies on the duration of life XIV: The comparative mortality of certain lower organism, Quart. Rev.Biology, 10: 60-79.
Comfort,A., (1964). Aging: Biology of Senescence, Routledge and Kegan, Paul, London.
Maynard-Smith, J., (1966). Theories of Aging, Topics in the biology of Aging, Wiley and Sons, New York, 1-27.
Hayflich, L., (1980). The cell biology of human aging, Scientific American, 422:42-49.
Bozcuk, A.N. (1976). Drosophila melanogaster (Diptera: Drosophilidae) yaşlanması ve Orgel hipotezi üzerine araştırmalar” Doçentlik tezi, Hacettepe Üniversitesi, Ankara.
Curtsinger, J.W. (2002). Sex specificity, life-span and statistical power, Journals of Gerontology Series A Biological Sciences and Medical Sciences, 57 (12): B409-414.
Nalçacı, O.B. (1984). Yaşlanma olayının Drosophila’da geroprotektörlerle ertelenmesi üzerine araştırmalar, Hacettepe Üniversitesi Fen Bilimleri Enstitüsü, Ankara.
Koç, Y. (1998). Fotoperiyot ve besin çeşidinin Drosophila melanogaster’in gelişim süresi, ergin hayat süresi, verim ve eşey oranına etkisi, Ondokuz Mayıs Üniversitesi Fen Bilimleri Enstitüsü, Samsun.
Ueyama, M. And Fuyama, Y. (2003). Enhanced cost of mating in female sterile mutants of Drosophila melanogaster, Genes & Genetic Systems, 78 (1): 29-36.
Prowse, N., and Partridge, L. (1997). The effects of reproduction on longevity and fertility in male Drosophila melanogaster, Journal of Insect Physiology, 43(6):501-512.
Arking, R., Buck, S., Novoseltev, V.N., Hwangbo, D.S. and Lane, M. (2002). Genomic plasticity, energy allocations, and the extended longevity phenotypes of Drosophila, Ageing Research Reviews, 1 (2): 209-228.
Novoseltsev, V.N., Novoseltseva, J.A., Boyko, S.I. and Yashin, A.I. (2003). What fecundity patterns indicate about aging and longevity: Insights from Drosophila studies, Journals of Gerontology Series A-Biological Sciences and Medical Sciences, 58 (6): 484-494.
David, J., Van Herrewege, J. and Fouillet, P., (1971). Quantitative under feeding of Drosophila effects on adult longevity and fecundity, Experimental Gerontology 6, 249-257.
Good, T.P. and Tatar, M. (2001). Age-specific mortality and reproduction respond to adult dietary restriction in Drosophila melanogaster, Journal of Insect Physiology, 47 1467-1473.
Carey, J.R., Liedo, P., Harshman, L., Zhang, Y., Muller, H.G., Partridge, L. and Wang, J.L. (2002). Life history response of Mediterranean fruit flies to dietary restriction, Aging Cell, 1 (2): 140-148.
Norry, F.M. and Loeschcke, V.R. (2002). Longevity and resistance to cold stress in cold-stress selected lines and their controls in Drosophila melanogaster, Journal of Evolutionary Biology, 15 (5): 775-783.
Economus, A.C. and Lints, F.A. (1986). Developmental temperature and life span in Drosophila melanogaster, Gerontology, 32 (1): 18-27.
Fred, H. and Timothy, J.B. (1997). An analysis of resouece allocation in response to dietary yeast in Drosophila melanogaster, Journal of Insect Physiology, 43(8): 779-788.