Özge TEMİZ, Tüzün AYTEKİN, Hikmet Yeter ÇOĞUN, Ferit KARGIN

Klorantraniliprol İndüklü Toksisitenin Oreochromis niloticus Solungaç İyon Taşınım Enzim Aktivitelerine Etkileri -

Diamid türü bir insektisit olan klorantraniliprol tarım uygulamalarında çok kullanılan, zararlılarda iyonoregülasyon sistemini bloke eden, toksik özelliği ile etkinlik gösteren bir pestisittir. Bu çalışmada klorantraniliprol, 1 ppm ve 2 ppm konsantrasyonlarının etkisinde Oreochromis niloticus solungaç dokularında iyon transport enzim aktivitelerine üzerine etklileri 7 gün süre ile araştırılmıştır. Araştırmamızda elde edilen sonuçlar solungaç dokusunda klorantraniliprol 7 gün süreyle etkisinde Na+ /K+ -ATPaz enzim aktivitesinin kontrol grubuna göre 1 ppm ve 2 ppm konsantrasyonlarında sırasıyla % 61 ve % 65 azalma gösterdiği belirlendi. Klorantraniliprol maruziyetinde, kontrol grubuna göre 1 ppm ve 2 ppm konsantrasyonlarında sırasıyla Ca2+-ATPaz enzim aktivitesinde %68 ve %76, Mg2+-ATPaz enzim aktivitesinde %28 ve %44 azalma olduğu belirlendi. Çalışmanın sonuçları, Oreochromis niloticus balıklarının iyon regülasyon sisteminin, klorantraniliprol’ün etkisinde iyi bir biyobelirteç olduğunu göstermektedir.

Effects of Chlorantraniliprole-Induced Toxicity on Oreochromis niloticus Gill Ion Transport Enzyme Activities

Chlorantraniliprole, a diamide type insecticide, is a pesticide that is very used in agricultural applications, in this sense, it causes toxicity of the ionoregulation system against pests and is effective with its toxic feature. In this study, the effects of chlorantraniliprole, 1ppm and 2 ppm concentrations on ion-transporting enzyme activity in Oreochromis niloticus gill tissues were investigated for 7 days. The results obtained in our study showed that Na+ /K+ - ATPase enzyme activity showed a 61% and 65% reduction in concentrations of 1 ppm and 2 ppm, respectively, in the effect of chlorantraniliprole in gill tissue for 7 days. In the exposure to chlorantraniliprole, it was determined that there was a 68% and 76% decrease in Ca2+- ATPase enzyme activity and 28% and 44% in Mg2+-ATPaz enzyme activity at concentrations of 1 ppm and 2 ppm compared to the control group. The results of the study show that the ion regulation system of Oreochromis niloticus are a biomarker in the effect of chlorantraniliprole.

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