Elif DEMİRKAN, Tuba SEVGİ, Aynur AYBEY, Halime KOCAKURT

Kırmızı Solucan Eisenia fetida Kaynaklı Sölomik Sıvının Bazı Biyolojik Özelliklerinin Araştırılması

Eisenia fetida, toprak ortamının bol bulunan hayati üyelerindendir. Salgıladıkları sölomik sıvı fizyolojik aktivitelerine yardımcı olur. Stres sırasında serbest bıraktıkları bu sıvı, toprak sağlığı ve ekotoksisite üzerinde de önemli bir etkiye sahiptir. Bu çalışmada, sölomik sıvının bazı biyolojik özellikleri araştırılmıştır. Bu amaçla sölomik sıvıdan 3 bakteri seçilmiş, 16S rRNA dizilimine göre tanımlanmış ve bunlardan 2 tanesi Bacillus cereus ve 1 tanesi Bacillus thuringiensis ile benzerlik göstermiştir. Sölomik sıvıdaki enzimatik aktiviteler kontrol edildiğinde lipaz, selülaz, amilaz, proteaz, fitaz ve arilsülfataz aktivitelerinin varlığı tespit edilmiştir. Sölomik sıvının antibakteriyel etkileri Enterococcus faecalis, Escherichia coli, Staphylococcus epidermidis, Stapylococcus aureus, Klebsiella pneumoniae, Salmonella typhimurium, Yersinia enterocolitica ve Pseudomonas aeruginosa’ya karşı araştırılmıştır. Sonuçlara göre sölom bakterileri üzerinde inhibitör etkiler göstermiştir. Buna ek olarak, ince tabaka kromatografisi sonuçları ise sölomik sıvıda glikoz ve maltoz varlığını göstermiştir. Sıvının protein paterni, 17-46 kDa aralığında beş açık protein bandı göstermiştir. Bu çalışma ile sölomik sıvının bazı biyolojik özellikleri ortaya konulmuş olup, bundan sonraki çalışmalara ışık tutacaktır.

Anahtar Kelimeler:

Eisenia fetida, Antimikrobiyal aktivite, Sölomik sıvı, Solucan, Eisenia fetida, Enzim aktivitesi, Protein profili, Eisenia fetida, Enzim aktivitesi, Protein profili

Investigation of Some Biological Properties of Coelomic Fluid Originating from the Red Worm Eisenia fetida

Eisenia fetida is abundant and vital member of the soil environment. The coelomic fluid they secrete help their physiological activities. This liquid, which they release during stress, also has a significant effect on soil health and ecotoxicity. In this study, some biological properties of coelomic fluid were investigated. For this purpose, 3 bacteria were selected from coelomic fluid, they were identified based on the 16S rRNA sequence and 2 of these were similar to Bacillus cereus and 1 to Bacillus thuringiensis. When the enzymatic activities in the coelomic fluid were controlled, the presence of lipase, cellulase, amylase, protease, phytase and arylsulfatase activities were detected. The antibacterial effects of the coelomic fluid was investigated against Enterococcus faecalis, Escherichia coli, Staphylococcus epidermidis, Staphylococcus aureus, Klebsiella pneumoniae, Salmonella typhimurium, Yersinia enterocolitica and Pseudomonas aeruginosa. According to the results, it showed inhibitory effects on test bacteria. Additionally, the thin layer chromatography results showed the presence of glucose and maltose in the coelomic fluid. Protein pattern of fluid showed five clear protein bands in the range of 17–46 kDa. With this study, some biological properties of coelomic fluid have been revealed, and will shed light on future studies.

Keywords:

Coelomic fluid, Earthworm, Enzyme activity Protein profile, Eisenia fetida, Antimicrobial activity, , Antimikrobiyal aktivite, Sölomik sıvı, Solucan, Eisenia fetida, Enzim aktivitesi, Protein profili,

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