Yıldız Gölü Sedimentinden İzole Edilen Aktinobakterilerin Antimikrobiyal ve Enzim Üretim Kapasitelerinin Araştırılması

Bu çalışmada, Yıldız Gölü (Gümüşhane) sedimentinden aktinobakteri izolasyonu gerçekleştirilmiş ve izolatlar antimikrobiyal aktivite ve enzim üretme kapasiteleri (amilaz, lipaz, proteaz, pektinaz, selülaz) bakımından incelenmiştir. Tarama, uygun besiyeriler içeren petrilerde gerçekleştirilmiştir. İzolasyon için SCA besiyeri kullanılmış ve besiyeriye nistatin ve nalidiksik asit ilave edilmiştir. Antimikrobiyal aktivite tespiti için, çapraz çizgi ekim yöntemi ve C. tropicalis, C. albicans, E. coli, P. aeruginosa, MRSA, E. faecium, E. fecalis standart suşları tercih edilmiştir. Sonuç olarak izolatların %55’i en az bir test mikroorganizmasına karşı antimikrobiyal aktivite gösterirken %66.6’sının ise en az bir enzimi üretebildiği belirlenmiştir. Bununla birlikte izolatların %22.2’si ne antimikrobiyal aktivite ne de enzim üretme yeteneğine sahip bulunmuştur. Bu çalışma Yıldız Gölü’nden aktinobakteri izolasyonu ve izolatların biyolojik aktivitesi üzerine gerçekleştirilen ilk araştırmadır. Sonuç olarak, elde edilen aktinobakterilerin endüstriyel ve farmakolojik çalışmalar için kaynak olabileceği öngörülmekle birlikte aktif bileşiklerin izolasyonu ve karakterizasyonu için ileri çalışmalara ihtiyaç duyulmaktadır

Anahtar Kelimeler:

Antimikrobiyal aktivite, Enzim üretimi, İzolasyon, Tarama

Investigation of Antimicrobial and Enzyme Production Capacities of Actinobacteria Isolated from Yıldız Lake Sediment

In this study, actinobacteria was isolated from Yıldız Lake (Gümüşhane) sediment and the isolates were examined for antimicrobial activity and enzyme production capacities (amylase, lipase, protease, pectinase, cellulase). Screening was carried out on petri dishes containing suitable media. SCA medium was used for isolation and nistatin and nalidixic acid were added. For the detection of antimicrobial activity cross-streak method and C. tropicalis, C. albicans, E. coli, P. aeruginosa, MRSA, E. faecium, E. fecalis standard strains were used. As a result, 55% of the isolates showed antimicrobial activity agains to at least one test microorganism while 66.6% were able to produce at least one enzyme. However, 22.2% of the isolates were not capable of producing either antimicrobial activity or enzyme. This study was the first study on isolation of actinobacteria from Yıldız Lake and the biological activity of isolates. As a results, it is expected that the actinobacteria obtained from this sediment may be the source for industrial and pharmacological studies, but further studies are needed for the isolation and characterization of the active compounds.

Keywords:

Antimicrobial activity, Enzyme production, Isolation, Screening,

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